Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei, China.
Bioengineered. 2021 Dec;12(1):7079-7092. doi: 10.1080/21655979.2021.1977053.
miR-186-3p acts as a tumor suppressor in various cancers. This study aimed to explore the expression levels of miR-186-3p and its role in cervical cancer. We analyzed the effects of miR-186-3p and insulin-like growth factor 1 (IGF1) on the proliferation, invasion, and apoptosis of cervical cancer cells in vitro by regulating the PI3K/Akt signaling pathway. In cervical cancer tissues and cells, miR-186-3p was downregulated, and IGF1 was upregulated. In addition, miR-186-3p inhibited cell proliferation and invasion and enhanced apoptosis of cervical cancer cells. Moreover, our results showed that miR-186-3p inversely regulated the mRNA expression of IGF1 through direct contact. Knockdown of IGF1 reversed the results of miR-186-3p inhibitor in cervical cancer cells. In addition, the PI3K/Akt signaling pathway was activated by the miR-186-3p inhibitor, although partially arrested by IGF1 knockdown. The PI3K/Akt signaling pathway inhibitor suppressed miR-186-3p inhibitor-stimulated cell proliferation in cervical cancer. In conclusion, miR-186-3p inhibits tumorigenesis of cervical cancer by repressing IGF1, which inactivates the PI3K/Akt signaling pathway, implicating miR-186-3p as a potential new target for the treatment of cervical cancer.
miR-186-3p 在多种癌症中充当肿瘤抑制因子。本研究旨在探讨 miR-186-3p 的表达水平及其在宫颈癌中的作用。我们通过调节 PI3K/Akt 信号通路，分析了 miR-186-3p 和胰岛素样生长因子 1（IGF1）对体外宫颈癌细胞增殖、侵袭和凋亡的影响。在宫颈癌组织和细胞中，miR-186-3p 下调，IGF1 上调。此外，miR-186-3p 抑制宫颈癌细胞的增殖和侵袭，增强细胞凋亡。而且，我们的结果表明，miR-186-3p 通过直接接触反向调节 IGF1 的 mRNA 表达。IGF1 的敲低逆转了 miR-186-3p 抑制剂在宫颈癌细胞中的作用。此外，PI3K/Akt 信号通路被 miR-186-3p 抑制剂激活，尽管部分被 IGF1 敲低阻滞。PI3K/Akt 信号通路抑制剂抑制了 miR-186-3p 抑制剂刺激的宫颈癌细胞增殖。总之，miR-186-3p 通过抑制 IGF1 抑制宫颈癌的肿瘤发生，从而使 PI3K/Akt 信号通路失活，表明 miR-186-3p 可能成为治疗宫颈癌的新靶点。
