Guenther Daniel, Liu Chaoxu, Horstmann Hauke, Krettek Christian, Jagodzinski Michael, Haasper Carl
Trauma Department, Hannover Medical School (MHH), Hannover, Germany.
Trauma Department, Hannover Medical School (MHH), Hannover, Germany ; Orthopaedic Department, HELIOS-ENDO-Klinik Hamburg, Hamburg, Germany.
Open Orthop J. 2014 May 16;8:93-9. doi: 10.2174/1874325001408010093. eCollection 2014.
Near-Infrared Spectroscopy (NIRS) could be of clinical relevance in modern cartilage regeneration.In a miniature pig model correlation of measurements and histologic scores have never been used before. The data analysis was part of an animal project that investigated the effects of seeding a chondrogenic and osteogenic scaffold with a bone-marrow-derived cell concentrate and reports the histological and mechanical properties. We created 20 osteochondral defects in the femoral condyles of 10 miniature pigs.The defects were left empty (E), filled with the grafted cylinder upside down (U), or with a combined scaffold (S) containing a spongy bone cylinder covered with a collagen membrane. In the fourth group, the same scaffolds were implanted but seeded with a stem cell concentrate (S+BMCC). The animals were euthanized after 3 months, and histologic and spectrometric analyses were performed. NIRS measurements were significantly higher in the central area of the defects of group S+BMCC compared to the central area of the defects of group U. In all groups, a correlation between NIRS and the histologic scores could be demonstrated though on different levels. In the central area, a good NIRS measurement correlates with low (good) histologic scores. In group E and group S, this negative correlation was significant (p=0.01). For the first time, NIRS was successfully used to evaluate osteochondral constructs in a miniature pig model.
近红外光谱（NIRS）在现代软骨再生中可能具有临床相关性。在小型猪模型中，此前从未使用过测量值与组织学评分的相关性研究。数据分析是一项动物项目的一部分，该项目研究了用骨髓来源的细胞浓缩物接种软骨生成和成骨支架的效果，并报告了其组织学和力学性能。我们在10只小型猪的股骨髁上制造了20个骨软骨缺损。缺损处保持空置（E组），或倒置植入移植圆柱体（U组），或植入含有覆盖胶原膜的松质骨圆柱体的组合支架（S组）。在第四组中，植入相同的支架，但接种了干细胞浓缩物（S+BMCC组）。3个月后对动物实施安乐死，并进行组织学和光谱分析。与U组缺损的中心区域相比，S+BMCC组缺损的中心区域的NIRS测量值显著更高。在所有组中，尽管程度不同，但均能证明NIRS与组织学评分之间存在相关性。在中心区域，良好的NIRS测量值与低（良好）组织学评分相关。在E组和S组中，这种负相关性显著（p=0.01）。首次成功地将NIRS用于评估小型猪模型中的骨软骨构建体。
