Department of Pediatrics, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan.
J Clin Biochem Nutr. 2014 May;54(3):190-7. doi: 10.3164/jcbn.13-74. Epub 2014 Apr 9.
Non-alcoholic fatty liver disease is the most common liver disorder in developed countries, and its incidence is increasing in all population groups. As an antioxidant, vitamin E is effective in the treatment of non-alcoholic fatty liver disease, although the mechanism is still unclear. Methionine-choline deficient Wistar rats (n = 5) used as an experimental model of non-alcoholic fatty liver disease were fed a vitamin E-enriched diet (500 mg/kg) for 4 weeks. The effects were assessed by measuring lipid peroxidation, α-tocopherol levels, and the expression of α-tocopherol-related proteins in the liver. In vitamin E-treated methionine-choline deficient rats, lipid peroxidation was reduced, but liver histopathological changes were not improved. Hepatic α-tocopherol levels in these rats were significantly elevated compared to normal rats treated with vitamin E. Expression of liver α-tocopherol transfer protein in vitamin E-treated methionine-choline deficient rats was significantly repressed compared to methionine-choline deficient rats. The expression of liver cytochrome P450 4F2 and ATP-binding cassette transporter protein 1, involved in metabolism and transport of α-tocopherol, respectively, was significantly repressed in vitamin E-treated methionine-choline deficient rats. In methionine-choline deficient rats, vitamin E treatment altered the hepatic α-tocopherol-related protein expression, which may affect α-tocopherol status in the liver, leading to reduced lipid peroxidation.
非酒精性脂肪性肝病是发达国家最常见的肝脏疾病，其在所有人群中的发病率都在增加。作为一种抗氧化剂，维生素 E 对非酒精性脂肪性肝病的治疗有效，尽管其机制尚不清楚。蛋氨酸-胆碱缺乏的 Wistar 大鼠（n = 5）被用作非酒精性脂肪性肝病的实验模型，用富含维生素 E 的饮食（500mg/kg）喂养 4 周。通过测量脂质过氧化、α-生育酚水平和肝脏中与α-生育酚相关的蛋白质的表达来评估效果。在接受维生素 E 治疗的蛋氨酸-胆碱缺乏的大鼠中，脂质过氧化减少，但肝组织病理学变化没有改善。与接受维生素 E 治疗的正常大鼠相比，这些大鼠的肝α-生育酚水平显著升高。与蛋氨酸-胆碱缺乏的大鼠相比，接受维生素 E 治疗的蛋氨酸-胆碱缺乏的大鼠肝脏α-生育酚转移蛋白的表达显著受到抑制。分别参与α-生育酚代谢和转运的肝细胞色素 P450 4F2 和 ATP 结合盒转运蛋白 1 的表达在接受维生素 E 治疗的蛋氨酸-胆碱缺乏的大鼠中显著受到抑制。在蛋氨酸-胆碱缺乏的大鼠中，维生素 E 治疗改变了肝脏α-生育酚相关蛋白的表达，这可能影响肝脏中α-生育酚的状态，导致脂质过氧化减少。
