Misaki Kiryo, Takitani Kimitaka, Ogihara Tohru, Inoue Akiko, Kawakami Chihiro, Kuno Tomoko, Kawamura Naohisa, Miyake Munenori, Nakagawa Toshimasa, Tamai Hiroshi
a Department of Pediatrics Osaka Medical College Daigakumachi Takatsuki 569-8686 Osaka Japan.
b Department of Clinical Pathology Osaka Medical College Daigakumachi Takatsuki 569-8686 Osaka Japan.
Free Radic Res. 2003;37(9):1037-1042. doi: 10.1080/10715760310001600426.
α-Tocopherol (a form of vitamin E) is a fat-soluble vitamin that can prevent lipid peroxidation of cell membranes. This antioxidant activity of α-tocopherol can help to prevent cardiovascular disease, atherosclerosis and cancer. We investigated the α-tocopherol level and the expression of α-tocopherol transfer protein (α-TTP) in the leukocytes of children with leukemia. The plasma and erythrocyte α-tocopherol levels did not differ between children with leukemia and the control group. However, lymphocytes from children with leukemia had significantly lower α-tocopherol levels than lymphocytes from the controls (58.4±39.0 ng/mg protein versus 188.9±133.6, respectively; p<0.05), despite the higher plasma α-tocopherol/cholesterol ratio in the leukemia group (5.83±1.64 μmol/mmol versus 4.34±0.96, respectively; p<0.05). No significant differences in the plasma and leukocyte levels of isoprostanes (the oxidative metabolites of arachidonic acid) were seen between the leukemia patients and controls. The plasma level of acrolein, a marker of oxidative stress, was also similar in the two groups. Investigation of α-TTP expression by leukocytes using real-time PCR showed no difference between the two groups. These findings suggest that there may be comparable levels of lipid peroxidation in children with untreated leukemia and controls, despite the reduced α-tocopherol level in leukemic leukocytes.
