Kawasaki A, Kawano K, Terada Y, Hirayasu R
Nihon Hotetsu Shika Gakkai Zasshi. 1989 Jun;33(3):522-7. doi: 10.2186/jjps.33.522.
The interaction of Hydroxyapatite (HAP) with organic molecules is biologically important. Some proteins display a high adsorption affinity for HAP and they have been implicated in the mineralization of bone. In order to understand the interaction of HAP with proteins, it is important to know which amino acid side-groups are involved in the protein-HAP interaction and what type of interaction takes place. We investigated the interaction of HAP with amino acids and their derivatives by crystal growth experiment. The rate of crystal growth was measured by adding seed crystals of HAP (synthetic apatite) to a solution of CaCl2 and KH2PO4, pH 7.4 at 37 degrees C. Basic amino acids, acidic amino acids and amino acids with polor side-groups inhibit the rate of crystal growth. These findings suggest that amino acid side-groups, such as carboxyl group, amino group, hydroxyl group and imidazole, are related to the affinity for HAP.
羟基磷灰石（HAP）与有机分子的相互作用具有重要的生物学意义。一些蛋白质对HAP表现出高吸附亲和力，并且它们与骨矿化有关。为了理解HAP与蛋白质的相互作用，了解哪些氨基酸侧链基团参与蛋白质-HAP相互作用以及发生何种类型的相互作用很重要。我们通过晶体生长实验研究了HAP与氨基酸及其衍生物的相互作用。通过在37℃将HAP（合成磷灰石）晶种添加到pH 7.4的CaCl2和KH2PO4溶液中来测量晶体生长速率。碱性氨基酸、酸性氨基酸和具有极性侧链基团的氨基酸会抑制晶体生长速率。这些发现表明，氨基酸侧链基团，如羧基、氨基、羟基和咪唑，与对HAP的亲和力有关。
