Department of Chemistry, Purdue University , West Lafayette, Indiana 47907, United States.
Anal Chem. 2014 Jul 1;86(13):6533-9. doi: 10.1021/ac501034v. Epub 2014 Jun 19.
Ion-molecule reactions provide a powerful tool for structural elucidation of ionized pharmaceutical analytes in tandem mass spectrometry. However, all previous interfaces for the introduction of reagents for ion-molecule reactions have utilized a single reagent approach. In this study, a multiported pulsed valve system was designed and characterized for rapid introduction of three neutral reagents into a linear quadrupole ion trap. Additionally, automatic triggering was used to allow for the introduction of the reagents on a chromatographic time scale. This system enables automatic, high throughput screening of complex mixtures by using at least three different ion-molecule reactions. Further, rapid testing of new neutral reagents is also possible.
离子-分子反应为串联质谱法中离子化药物分析物的结构阐明提供了强大的工具。然而，以前所有用于引入离子-分子反应试剂的接口都采用了单一试剂方法。在这项研究中，设计并表征了一种多端口脉冲阀系统，用于将三种中性试剂快速引入线性四极离子阱中。此外，还使用自动触发来允许在色谱时间尺度上引入试剂。该系统通过使用至少三种不同的离子-分子反应，实现了对复杂混合物的自动、高通量筛选。此外，还可以快速测试新的中性试剂。
