Department of Chemistry , Purdue University , West Lafayette , Indiana 47907 , United States.
Anal Chem. 2019 Dec 17;91(24):15652-15660. doi: 10.1021/acs.analchem.9b03768. Epub 2019 Nov 21.
Gas-phase ion/molecule reactions have been used extensively for the structural elucidation of organic compounds in tandem mass spectrometry. Reagents for ion/molecule reactions can be introduced into a mass spectrometer via a continuous flow apparatus or through a pulsed inlet system. However, most of these approaches enable the use of only a single reagent at a time. In this work, a multichannel pulsed-valve inlet system was developed for the rapid consecutive introduction of up to nine different reagents or reagent systems into a linear quadrupole ion trap mass spectrometer for diagnostic gas-phase ion/molecule reactions. Automated triggering of the pulsed valves enabled these experiments to be performed on the high-performance liquid chromatography (HPLC) time scale. This enables high-throughput screening of several functionalities in analytes as they elute from an HPLC column.
气相离子/分子反应已广泛用于串联质谱法中有机化合物的结构阐明。离子/分子反应的试剂可以通过连续流装置或通过脉冲入口系统引入质谱仪。然而，这些方法中的大多数一次只能使用一种试剂。在这项工作中，开发了一种多通道脉冲阀入口系统，用于将多达九种不同的试剂或试剂系统快速连续地引入线性四极离子阱质谱仪中，以进行诊断气相离子/分子反应。脉冲阀的自动触发使这些实验能够在高效液相色谱 (HPLC) 的时间尺度上进行。这使得可以在 HPLC 柱洗脱时对分析物中的几个功能进行高通量筛选。
