Zin Noraziah Mohamad, Chit Yong Mei, Abu Bakar Nur Faizah
Pak J Biol Sci. 2014 Feb 1;17(3):356-63. doi: 10.3923/pjbs.2014.356.363.
The increment of rate in obesity, the phenomenon of fat phobia as well as the increased use of herbal medicine had lead to the emergence of herbal slimming products. However, numerous bacteria and heavy metal contaminations are often found in herbal products due to irregular handling practices. Ten different brands of products (labeled as A-J) were investigated. Seven heavy metals content such as As, Cd, Pb, Co, Cr, Cu and Zn were analyzed using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) and bacterial presence was determined by counting the total aerobic count. The identification of isolates was carried out by macroscopic and microscopic observation, biochemical tests and confirmation using commercial kits of Microgen GN-ID A+B and API 20 E. The heavy metal contents in the samples were below the limit of the standard limitation by WHO and Health Canada. However, sample A contained the highest total daily intake of heavy metals. Total aerobic count was highest in sample H followed by G, A, B, C, F, D, E, I and J in which G and H exceeded the standard total aerobic count (10(5) CFU g(-1)) as given by WHO. A total of nine isolates of Bacillus spp. and ten gram-negative bacteria were isolated in which Bacillus cereus and Pseudomonas aeruginosa were found in samples C and F, respectively. Considering the fact that the herbal sliming products contained low concentration of heavy metals and bacteria count, it should be consumed with caution.
肥胖率的上升、对脂肪的恐惧现象以及草药使用的增加导致了草药减肥产品的出现。然而，由于处理方式不当，草药产品中经常发现大量细菌和重金属污染。对十个不同品牌的产品（标记为A - J）进行了调查。使用电感耦合等离子体质谱法（ICP - MS）分析了七种重金属含量，如砷、镉、铅、钴、铬、铜和锌，并通过计算总需氧菌数来确定细菌的存在。通过宏观和微观观察、生化试验以及使用Microgen GN - ID A + B和API 20 E商业试剂盒进行确认来鉴定分离株。样品中的重金属含量低于世界卫生组织（WHO）和加拿大卫生部的标准限值。然而，样品A的重金属总日摄入量最高。总需氧菌数在样品H中最高，其次是G、A、B、C、F、D、E、I和J，其中G和H超过了WHO规定的标准总需氧菌数（10(5) CFU g(-1)）。总共分离出九株芽孢杆菌属菌株和十株革兰氏阴性菌，其中在样品C和F中分别发现了蜡样芽孢杆菌和铜绿假单胞菌。考虑到草药减肥产品中重金属浓度和细菌数量较低，仍应谨慎食用。
