Akimoto Y, Kaneko K, Fujii A
Nichidai Koko Kagaku. 1989 Sep;15(3):338-41.
Thirty-three strains of methicillin-resistant Staphylococcus aureus (MRSA, MIC for methicillin greater than or equal to 16.0 micrograms/ml) isolated from sputum were examined for their susceptibilities against 8 antimicrobial agents. Ampicillin (ABPC), methicillin (DMPPC), cefazolin (CEZ), minocycline (MINO), erythromycin (EM), tobramycin (TOB), refampicin (RFP), and ofloxacin (OFLX) were used as the antimicrobial agents. The production of beta-lactamase was also determined. The results are as follows: 1) The highest susceptibility was given by RFP, and followed by MINO, and OFLX. All strains were resistant against EM and TOB. 2) The percentage of beta-lactamase-positive strains was 75.8%, which was distributed nearly identical among each MIC to DMPPC (72.0-77.8%). 3) Since the beta-lactamase-positive strains were found among the sensitive strains to ABPC, it was suggested that the use of beta-lactam antimicrobial agents might bring into the higher MIC value to ABPC. 4) Since there were beta-lactamase-negative strains among the resistant strains to ABPC, it was indicated that penicillin binding protein-2' might be produced by giving beta-lactam antimicrobial agents.
对从痰液中分离出的33株耐甲氧西林金黄色葡萄球菌（MRSA，对甲氧西林的最低抑菌浓度大于或等于16.0微克/毫升）进行了8种抗菌药物的敏感性检测。使用氨苄西林（ABPC）、甲氧西林（DMPPC）、头孢唑林（CEZ）、米诺环素（MINO）、红霉素（EM）、妥布霉素（TOB）、利福平（RFP）和氧氟沙星（OFLX）作为抗菌药物。同时还测定了β-内酰胺酶的产生情况。结果如下：1）RFP的敏感性最高，其次是MINO和OFLX。所有菌株对EM和TOB均耐药。2）β-内酰胺酶阳性菌株的比例为75.8%，在每个对DMPPC的最低抑菌浓度（72.0 - 77.8%）之间分布几乎相同。3）由于在对ABPC敏感的菌株中发现了β-内酰胺酶阳性菌株，提示使用β-内酰胺类抗菌药物可能会使对ABPC的最低抑菌浓度升高。4）由于在对ABPC耐药的菌株中存在β-内酰胺酶阴性菌株，表明给予β-内酰胺类抗菌药物可能会产生青霉素结合蛋白-2'。
