Haller I, Heinrich S
Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1982 Apr;251(4):479-86.
The actual resistance of Staphylococcus aureus from different clinics in Bielefeld was examined on the basis of 155 strains freshly isolated from clinical specimens during a period of two months. Sensitivity of these strains was assessed by minimum inhibitory concentrations (MICs) as determined by agar dilution method. As expected oxacillin proved to be the most effective compound according to its stability to beta-lactamases. Ampicillin and amoxicillin were nearly as active as penicillin G, while propicillin exhibited a higher activity than penicillin G against those strains which were moderately sensitive to penicillin G. Mics of erythromycin were similar to those of propicillin and most of the highly penicillin resistant strains proved to be resistant to erythromycin too. When comparing the acylureidopenicillins and ticarcillin nearly identical MIC values resulted for azlocillin, mezlocillin and piperacillin; ticarcillin was found to be slightly less active. The qualitative assay for beta-lactamase activity performed after induction with methicillin demonstrated strong correlation between presence or absence of beta-lactamases and sensitivity of the bacteria to penicillin G. beta-lactamase activity could be found in almost all strains with MIC greater than or equal to 0.25 micrograms/ml for penicillin G. These results are discussed with respect to the recommendations for susceptibility testing.
基于在两个月内从临床标本中新鲜分离出的155株金黄色葡萄球菌，对来自比勒费尔德不同诊所的金黄色葡萄球菌的实际耐药性进行了检测。通过琼脂稀释法测定的最低抑菌浓度（MIC）来评估这些菌株的敏感性。正如预期的那样，根据其对β-内酰胺酶的稳定性，苯唑西林被证明是最有效的化合物。氨苄西林和阿莫西林的活性与青霉素G几乎相同，而丙匹西林对那些对青霉素G中度敏感的菌株表现出比青霉素G更高的活性。红霉素的MIC与丙匹西林相似，并且大多数对青霉素高度耐药的菌株也被证明对红霉素耐药。当比较酰脲基青霉素和替卡西林时，阿洛西林、美洛西林和哌拉西林的MIC值几乎相同；发现替卡西林的活性略低。用甲氧西林诱导后进行的β-内酰胺酶活性定性测定表明，β-内酰胺酶的存在与否与细菌对青霉素G的敏感性之间存在很强的相关性。在几乎所有对青霉素G的MIC大于或等于0.25微克/毫升的菌株中都能发现β-内酰胺酶活性。根据药敏试验的建议对这些结果进行了讨论。
