Sánchez-Siles Mariano, Camacho-Alonso Fabio, Ros-Llor Irene, López-Jornet Pia
Department of Oral Medicine, University of Murcia, Murcia, Spain; Department of Dentistry, University of Murcia, Murcia, Spain.
Int J Dermatol. 2014 Dec;53(12):1454-9. doi: 10.1111/ijd.12223. Epub 2014 Jun 5.
A study is made of DNA damage and apoptosis in a group of patients with oral leukoplakia (OL) with mild dysplasia.
The study comprised 30 patients with a clinicopathological diagnosis of OL with mild dysplasia and 30 controls. Both samples were similar in terms of age and gender distribution. Brush samples of lesion epithelial cells were collected, followed by cell centrifugation, preparation of the slides, fixation and staining, and analysis under the fluorescent light microscope. The exfoliated cells were examined to detect micronuclei (MN), nuclear buds, binucleated cells, condensed chromatin, pyknosis, and cells with karyorrhexis and karyolysis.
The patients with OL with mild dysplasia showed a greater frequency of MN (P < 0.001), nuclear buds (P = 0.018), and binucleated cells (P = 0.008).
Cytogenetic biomonitoring is a simple and scantly invasive technique allowing clinicians to assess DNA damage and apoptosis in patients with OL.
Oral cancer should be detected and controlled in its precancerous stages in order to increase survival rates. Leukoplakia lesions must be biomonitorized periodically. Biomonitorization offers sensibility, no morbidity, speed, and low cost.
对一组轻度发育异常的口腔白斑（OL）患者的DNA损伤和细胞凋亡进行研究。
该研究包括30例经临床病理诊断为轻度发育异常的OL患者和30例对照。两组样本在年龄和性别分布方面相似。采集病变上皮细胞的刷片样本，随后进行细胞离心、玻片制备、固定和染色，并在荧光显微镜下分析。检查脱落细胞以检测微核（MN）、核芽、双核细胞、凝聚染色质、核固缩以及核溶解和核碎裂的细胞。
轻度发育异常的OL患者显示出更高频率的MN（P < 0.001）、核芽（P = 0.018）和双核细胞（P = 0.008）。
细胞遗传学生物监测是一种简单且侵入性小的技术，可使临床医生评估OL患者的DNA损伤和细胞凋亡。
为提高生存率，应在口腔癌的癌前阶段对其进行检测和控制。必须定期对白斑病变进行生物监测。生物监测具有敏感性、无发病率、速度快和成本低的特点。
