嗜铬粒蛋白可以在猫和狗的样本中进行测量。
Chromogranins can be measured in samples from cats and dogs.
作者信息
Stridsberg Mats, Pettersson Ann, Hagman Ragnvi, Westin Christoffer, Höglund Odd
机构信息
Department of Medical Sciences, Uppsala University, SE-751 85 Uppsala, Sweden.
出版信息
BMC Res Notes. 2014 Jun 4;7:336. doi: 10.1186/1756-0500-7-336.
BACKGROUND
Methods for objective evaluation of stress in animals are important, but clinically difficult. An alternative method to study the sympathetic activity may be to investigate Chromogranin A (CGA), Chromogranin B (CGB) and Secretogranin II (SG2). The aim of this study was to investigate the cross-reactivity of CGA, CGB and SG2 between man, cat and dog and to explore possibilities to measure these proteins in samples from cats and dogs.
RESULTS
Adrenal gland extracts from feline and canine species were measured by region-specific radioimmunoassays in different dilution steps to explore possible inter species cross reactivity. High cross reactivity was found for cats in the CGA17-38, CGA324-337, CGA361-372, CGB and SG2 assays. High cross reactivity was found for dogs in the CGA17-38, CGA361-372, CGB and SN assays. The method measuring the intact CGA was not useful for measurements in cats and dogs.
CONCLUSIONS
Region-specific assays measuring defined parts of CGA, CGB and SG2 can be used for measurements in samples from cats and dogs. These results are promising and will allow for further studies of these proteins as possible clinical biomarkers in cats and dogs.
背景
客观评估动物应激的方法很重要，但在临床上具有难度。研究交感神经活动的一种替代方法可能是研究嗜铬粒蛋白A（CGA）、嗜铬粒蛋白B（CGB）和分泌粒蛋白II（SG2）。本研究的目的是调查CGA、CGB和SG2在人、猫和狗之间的交叉反应性，并探索在猫和狗的样本中测量这些蛋白质的可能性。
结果
通过区域特异性放射免疫分析法在不同稀释步骤下测量猫科和犬科动物的肾上腺提取物，以探索可能的种间交叉反应性。在CGA17 - 38、CGA324 - 337、CGA361 - 372、CGB和SG2检测中发现猫具有高交叉反应性。在CGA17 - 38、CGA361 - 372、CGB和SN检测中发现狗具有高交叉反应性。测量完整CGA的方法对猫和狗的测量无用。
结论
测量CGA、CGB和SG2特定部分的区域特异性检测方法可用于猫和狗样本的测量。这些结果很有前景，将有助于进一步研究这些蛋白质作为猫和狗可能的临床生物标志物。
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