Chappell Len, Killian Mary Lea, Spackman Erica
Georgia Poultry Laboratory Network, Oak wood, GA, USA.
Methods Mol Biol. 2014;1161:151-67. doi: 10.1007/978-1-4939-0758-8_14.
ELISA assays are a fast and relatively inexpensive way to screen sera for antibodies to avian influenza virus. Commercial ELISA kits are available, and although they are more expensive, they provide a ready-to-use assay with good quality control. Various sample types can be processed for ELISA: serum, plasma, egg yolk, blood collected on filter paper. Quality samples are critical to accurate results. The basics of AIV antibody ELISA, sample processing, results interpretation, and troubleshooting are discussed.
酶联免疫吸附测定（ELISA）是一种快速且相对经济的方法，用于筛查血清中针对禽流感病毒的抗体。有商业化的ELISA试剂盒可供使用，尽管它们价格更高，但提供了即用型检测且具有良好的质量控制。ELISA可处理多种样本类型：血清、血浆、蛋黄、滤纸上采集的血液。高质量的样本对于获得准确结果至关重要。本文将讨论禽流感病毒抗体ELISA的基本原理、样本处理、结果解读及故障排除。
