Exotic and Emerging Avian Viral Diseases Unit, US National Poultry Research Center, U.S. Department of Agriculture, Agricultural Research Service, Athens, GA, USA.
Avian Viruses Section, Diagnostic Virology Laboratory, National Veterinary Services Laboratories, US Department of Agriculture, Ames, IA, USA.
Methods Mol Biol. 2020;2123:177-193. doi: 10.1007/978-1-0716-0346-8_14.
ELISA assays are a fast and relatively inexpensive way to screen sera for antibodies to avian influenza virus. Commercial ELISA kits are available, and although they are more expensive than in-house tests, they provide a ready-to-use assay with good quality control. Numerous sample types can be processed for ELISA: serum, plasma, egg yolk, or blood collected on filter paper. High-quality samples are critical to accurate results. The basics of AIV antibody ELISA, sample processing, result interpretation, and troubleshooting are discussed.
ELISA 检测法是一种快速且相对廉价的方法，可用于筛选针对禽流感病毒的血清抗体。市售的 ELISA 试剂盒虽然比内部检测更为昂贵，但提供了即用型检测方法和良好的质量控制。可通过 ELISA 处理多种类型的样本：血清、血浆、蛋黄或在滤纸上采集的血液。高质量的样本对于准确的结果至关重要。本文讨论了 AIV 抗体 ELISA 的基础知识、样本处理、结果解读和故障排除。
