Biotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, GA, USA.
Department of Medicine, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.
Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):2944-2948. doi: 10.1099/ijs.0.057778-0. Epub 2014 Jun 4.
A polyphasic study was undertaken to determine the taxonomic position of 13 Campylobacter fetus-like strains from humans (n = 8) and reptiles (n = 5). The results of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS and genomic data from sap analysis, 16S rRNA gene and hsp60 sequence comparison, pulsed-field gel electrophoresis, amplified fragment length polymorphism analysis, DNA-DNA hybridization and whole genome sequencing demonstrated that these strains are closely related to C. fetus but clearly differentiated from recognized subspecies of C. fetus. Therefore, this unique cluster of 13 strains represents a novel subspecies within the species C. fetus, for which the name Campylobacter fetus subsp. testudinum subsp. nov. is proposed, with strain 03-427(T) ( = ATCC BAA-2539(T) = LMG 27499(T)) as the type strain. Although this novel taxon could not be differentiated from C. fetus subsp. fetus and C. fetus subsp. venerealis using conventional phenotypic tests, MALDI-TOF MS revealed the presence of multiple phenotypic biomarkers which distinguish Campylobacter fetus subsp. testudinum subsp. nov. from recognized subspecies of C. fetus.
进行了多相研究，以确定来自人类（n=8）和爬行动物（n=5）的 13 株类似弯曲杆菌胎儿的分类地位。基质辅助激光解吸电离飞行时间（MALDI-TOF）MS 和 sap 分析的基因组数据、16S rRNA 基因和 hsp60 序列比较、脉冲场凝胶电泳、扩增片段长度多态性分析、DNA-DNA 杂交和全基因组测序的结果表明，这些菌株与弯曲杆菌胎儿密切相关，但与弯曲杆菌胎儿的公认亚种明显不同。因此，这 13 株独特的菌株代表了弯曲杆菌胎儿种内的一个新亚种，建议将其命名为弯曲杆菌胎儿亚种。testudinum 亚种。nov.，其模式株为 03-427（T）（=ATCC BAA-2539（T）=LMG 27499（T））。尽管这个新分类群不能通过常规表型测试与弯曲杆菌胎儿亚种。胎儿和弯曲杆菌胎儿亚种。venerealis 区分开来，但 MALDI-TOF MS 显示出存在多个表型生物标志物，可将弯曲杆菌胎儿亚种。testudinum 亚种。nov. 与弯曲杆菌胎儿的公认亚种区分开来。
