通过Cy5.5-膜联蛋白V成像心肌缺血再灌注损伤。
Imaging Myocardial Ischemia and Reperfusion Injury via Cy5.5-Annexin V.
作者信息
Tian Rong, Pan DongFeng
机构信息
Department of Nuclear Medicine, Sichuan University, 37 Guoxuexiang, Chengdu, China 610041.
Radiology Department, University of Virginia, P.O. Box 800170, Charlottesville, VA 22908 USA.
出版信息
Nucl Med Mol Imaging. 2012 Sep;46(3):155-61. doi: 10.1007/s13139-012-0140-1. Epub 2012 May 15.
AIM
The aim of this article is to present the results of an imaging study of myocardial apoptosis induced by ischemia/reperfusion injury.
METHODS
Twenty nude mice were randomly divided into an experimental group (10 mice) and control group (10 mice). In the experimental group, myocardial apoptosis was induced by ligation of the left anterior descending coronary artery (LAD) for 30 min. This was followed by reperfusion for 90 min. In the control group, the heart was exposed for the same length of time as in the experimental group. Cy5.5-annexin V (25 μg) was injected into both sets of mice after the onset of reperfusion. At 90 min post-injection, the mice were imaged. The region of interest (ROI) was obtained, and the fluorescence intensity of the ROI was quantified. The animals were sacrificed, and myocardial apoptosis was assayed by TUNEL assay.
RESULTS
Fluorescence intensity in the ischemia/reperfusion hearts was significantly higher than that in the control group (P < 0.05). In the TUNEL assay, more apoptotic cells were observed in the experimental group than in the control group, correlating with imaging results.
CONCLUSION
Fluorescence imaging of Cy5.5-annexin V in a mouse model of myocardial ischemia/reperfusion can be used in vivo as a noninvasive means of detecting ischemia/reperfusion-induced apoptotic cells in the heart.
目的
本文旨在呈现一项关于缺血/再灌注损伤诱导心肌细胞凋亡的成像研究结果。
方法
将20只裸鼠随机分为实验组（10只）和对照组（10只）。在实验组中，通过结扎左冠状动脉前降支（LAD）30分钟诱导心肌细胞凋亡，随后再灌注90分钟。在对照组中，心脏暴露时间与实验组相同。再灌注开始后，向两组小鼠注射Cy5.5-膜联蛋白V（25μg）。注射后90分钟，对小鼠进行成像。获取感兴趣区域（ROI），并对ROI的荧光强度进行定量分析。处死动物，通过TUNEL法检测心肌细胞凋亡情况。
结果
缺血/再灌注心脏的荧光强度显著高于对照组（P < 0.05）。在TUNEL检测中，实验组观察到的凋亡细胞比对照组更多，与成像结果相符。
结论
在心肌缺血/再灌注小鼠模型中，Cy5.5-膜联蛋白V的荧光成像可作为一种非侵入性手段，用于在体内检测心脏中缺血/再灌注诱导的凋亡细胞。
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