Ramos Christian G, Grilo André M, Sousa Sílvia A, Feliciano Joana R, da Costa Paulo J P, Leitão Jorge H
Department of Bioengineering and Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.
PLoS One. 2014 Jun 5;9(6):e98813. doi: 10.1371/journal.pone.0098813. eCollection 2014.
Small non-coding RNAs (sRNAs) are important players of gene expression regulation in bacterial pathogens. MtvR is a 136-nucleotide long sRNA previously identified in the human pathogen Burkholderia cenocepacia J2315 and with homologues restricted to bacteria of the Burkholderia cepacia complex. In this work we have investigated the effects of expressing MtvR in Escherichia coli and Pseudomonas aeruginosa. Results are presented showing that MtvR negatively regulates the hfq mRNA levels in both bacterial species. In the case of E. coli, this negative regulation is shown to involve binding of MtvR to the 5'-UTR region of the hfqEc mRNA. Results presented also show that expression of MtvR in E. coli and P. aeruginosa originates multiple phenotypes, including reduced resistance to selected stresses, biofilm formation ability, and increased susceptibility to various antibiotics.
小非编码RNA（sRNA）是细菌病原体中基因表达调控的重要参与者。MtvR是一种长度为136个核苷酸的sRNA，先前在人类病原体洋葱伯克霍尔德菌J2315中被鉴定出来，其同源物仅限于洋葱伯克霍尔德菌复合体的细菌。在这项工作中，我们研究了在大肠杆菌和铜绿假单胞菌中表达MtvR的影响。结果表明，MtvR在这两种细菌中均对hfq mRNA水平产生负调控作用。在大肠杆菌中，这种负调控作用涉及MtvR与hfqEc mRNA的5'-UTR区域结合。结果还表明，在大肠杆菌和铜绿假单胞菌中表达MtvR会产生多种表型，包括对特定应激的抗性降低、生物膜形成能力下降以及对各种抗生素的敏感性增加。
