Mohammadi Parvin, Kalantar Enayatollah, Bahmani Nasrin, Fatemi Adel, Naseri Nima, Ghotbi Nahid, Naseri Mohammad Hassan
Department of Midwifery and Nursing, Faculty of Midwifery and Nursing, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
Department of Microbiology and Immunology, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran
Acta Med Iran. 2014;52(5):337-40.
Bacteremia continues to result in significant morbidity and mortality, particularly among neonates. There is scarce data on neonatal bacteremia in among Iranian neonates. In this study, we determined neonatal bacteremia isolates and their antibiotic resistance pattern in neonatal insensitive care unit at Beasat hospital, Sanandaj, Iran. During one year, all neonates admitted to the NICU were evaluated. Staphylococcal isolates were subjected to determine the prevalence of MRS and mecA gene. A total of 355 blood cultures from suspected cases of sepsis were processed, of which 27 (7.6%) were positive for bacterial growth. Of the 27 isolates, 20 (74%) were Staphylococcus spp as the leading cause of bacteremia. The incidence of Gram negative bacteria was 04 (14.8%). The isolated bacteria were resistant to commonly used antibiotics. Maximum resistance among Staphylococcus spp was against Penicillin, and Ampicillin. In our study, the isolated bacteria were 7.5 % Vancomycin and Ciprofloxacin sensitive. Oxacillin disk diffusion and PCR screened 35% and 30% mec a positive Staphylococcus spp. The spectrum of neonatal bacteremia as seen in NICU at Beasat hospital confirmed the importance of pathogens such as Staphylococcus spp. Penicillin, Ampicillin and Cotrimoxazol resistance was high in theses isolates with high mecA gene carriage, probably due to antibiotic selection.
菌血症仍然会导致显著的发病率和死亡率，尤其是在新生儿中。关于伊朗新生儿菌血症的数据很少。在本研究中，我们确定了伊朗桑纳达杰市贝萨特医院新生儿重症监护病房中新生儿菌血症的分离菌株及其抗生素耐药模式。在一年时间里，对所有入住新生儿重症监护病房的新生儿进行了评估。对葡萄球菌分离株进行了耐甲氧西林葡萄球菌（MRS）和mecA基因流行率的测定。共处理了355份疑似败血症病例的血培养样本，其中27份（7.6%）细菌生长呈阳性。在这27株分离菌株中，20株（74%）为葡萄球菌属，是菌血症的主要原因。革兰氏阴性菌的发生率为4株（14.8%）。分离出的细菌对常用抗生素耐药。葡萄球菌属中对青霉素和氨苄西林的耐药性最高。在我们的研究中，分离出的细菌对万古霉素和环丙沙星的敏感性为7.5%。苯唑西林纸片扩散法和聚合酶链反应（PCR）检测出35%和30%的葡萄球菌属mecA基因呈阳性。贝萨特医院新生儿重症监护病房中所见的新生儿菌血症谱证实了葡萄球菌属等病原体的重要性。这些分离菌株中青霉素、氨苄西林和复方新诺明的耐药性很高，且mecA基因携带率高，这可能是由于抗生素的选择所致。
