a Department of Plant Systems Biology, VIB, Ghent University, B-9052 Gent, Belgium b Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium.
d VIB Compound Screening Facility, B-9052 Gent, Belgium.
Mol Plant. 2014 Jul;7(7):1138-50. doi: 10.1093/mp/ssu071. Epub 2014 Jun 5.
Mitochondria are crucial players in the signaling and metabolic homeostasis of the plant cell. The molecular components that orchestrate the underlying processes, however, are largely unknown. Using a chemical biology approach, we exploited the responsiveness of Arabidopsis UDP-glucosyltransferase-encoding UGT74E2 towards mitochondrial perturbation in order to look for novel mechanisms regulating mitochondria-to-nucleus communication. The most potent inducers of UGT74E2 shared a (2-furyl)acrylate (FAA) substructure that negatively affected mitochondrial function and was identified before as an auxin transcriptional inhibitor. Based on these premises, we demonstrated that perturbed mitochondria negatively affect the auxin signaling machinery. Moreover, chemical perturbation of polar auxin transport and auxin biosynthesis was sufficient to induce mitochondrial retrograde markers and their transcript abundance was constitutively elevated in the absence of the auxin transcriptional activators ARF7 and ARF19.
线粒体是植物细胞信号转导和代谢稳态的关键参与者。然而，协调这些过程的分子成分在很大程度上是未知的。我们使用化学生物学方法，利用拟南芥 UDP-葡萄糖基转移酶编码基因 UGT74E2 对线粒体扰动的反应性，以寻找调节线粒体到细胞核通讯的新机制。最能诱导 UGT74E2 的物质都含有（2-糠基）丙烯酰胺（FAA）亚结构，该亚结构会对线粒体功能产生负面影响，并在此前被鉴定为生长素转录抑制剂。基于这些前提，我们证明了失调的线粒体会对生长素信号机制产生负面影响。此外，对极性生长素运输和生长素生物合成的化学干扰足以诱导线粒体逆行标记物，并且在缺乏生长素转录激活因子 ARF7 和 ARF19 的情况下，其转录丰度持续升高。
