Kolonics Attila, Csiszovszki Zsolt, Tőke Enikő R, Lőrincz Orsolya, Haluszka Dóra, Szipőcs Róbert
Institute for Solid State Physics and Optics of Wigner RCP, Budapest, Hungary; R&D Ultrafast Lasers Ltd, Budapest, Hungary.
Exp Dermatol. 2014 Aug;23(8):596-605. doi: 10.1111/exd.12464.
Epidermal Langerhans cells (LCs) function as professional antigen-presenting cells of the skin. We investigated the LC-targeting properties of a special mannose-moiety-coated pathogen-like synthetic nanomedicine DermaVir (DV), which is capable to express antigens to induce immune responses and kill HIV-infected cells. Our aim was to use multiphoton laser microscopy (MLM) in vivo in order to visualize the uptake of Alexa-labelled DV (AF546-DV) by LCs. Knock-in mice expressing enhanced green fluorescent protein (eGFP) under the control of the langerin gene (CD207) were used to visualize LCs. After 1 h, AF546-DV penetrated the epidermis and entered the eGFP-LCs. The AF546-DV signal was equally distributed inside the LCs. After 9 h, we observed AF546-DV signal accumulation that occurred mainly at the cell body. We demonstrated in live animals that LCs picked up and accumulated the nanoparticles in the cell body.
表皮朗格汉斯细胞（LCs）作为皮肤的专职抗原呈递细胞发挥作用。我们研究了一种特殊的甘露糖部分包被的病原体样合成纳米药物DermaVir（DV）的LC靶向特性，该药物能够表达抗原以诱导免疫反应并杀死HIV感染细胞。我们的目的是在体内使用多光子激光显微镜（MLM），以便可视化LCs对Alexa标记的DV（AF546-DV）的摄取。利用在朗格汉斯蛋白基因（CD207）控制下表达增强型绿色荧光蛋白（eGFP）的敲入小鼠来可视化LCs。1小时后，AF546-DV穿透表皮并进入eGFP-LCs。AF546-DV信号在LCs内均匀分布。9小时后，我们观察到AF546-DV信号主要在细胞体处积累。我们在活体动物中证明，LCs在细胞体内摄取并积累了纳米颗粒。
