DSM Biotechnology Center, Alexander Fleminglaan 1, 2613 AX, Delft, The Netherlands,
Appl Microbiol Biotechnol. 2014;98(14):6215-31. doi: 10.1007/s00253-014-5799-z. Epub 2014 Jun 6.
Industry has an increasing interest in the use of enzymes as environmentally friendly, highly efficient, and specific bio-catalysts. Enzymes have primarily evolved to function in aqueous environments at ambient temperature and pressure. These conditions however do not always correspond with industrial processes or applications, and only a small portion of all known enzymes are therefore suitable for industrial use. Protein engineering can sometimes be applied to convey more desirable properties to enzymes, such as increased stability, but is limited to the 20 naturally occurring amino acids or homologs thereof. Using post-production modification, which has the potential to combine desirable properties from the enzyme and the conjugated compounds, enzymes can be modified with both natural and synthetic molecules. This offers access to a myriad of possibilities for tuning the properties of enzymes. At this moment, however, the effects of post-production modification cannot yet be reliably predicted. The increasing number of applications will improve this so that the potential of this technology can be fully exploited. This review will focus on post-production modification of enzymes and its use and opportunities in industry.
工业界越来越感兴趣地将酶用作环保、高效和特定的生物催化剂。酶主要是为了在环境温度和压力下的水相环境中发挥作用而进化的。然而，这些条件并不总是与工业过程或应用相对应，因此只有一小部分已知的酶适合工业用途。蛋白质工程有时可用于赋予酶更理想的性质，例如提高稳定性，但仅限于 20 种天然存在的氨基酸或其同源物。通过后生产修饰，可以将酶和缀合化合物的理想性质结合起来，酶可以用天然和合成分子进行修饰。这为调整酶的性质提供了无数的可能性。然而，目前，后生产修饰的效果尚无法可靠预测。随着应用的日益增多，这种情况将会得到改善，从而充分利用这项技术的潜力。本文将重点介绍酶的后生产修饰及其在工业中的应用和机遇。
