孟加拉玫瑰红与间接免疫荧光联合应用于布鲁氏菌病的诊断

[Combined use of rose Bengal and indirect immunofluorescence in the diagnosis of brucellosis].

作者信息

Colmenero J D, Reguera J M, Cabrera F P, Hernández S, Porras J, Manchado P, Miranda M T

出版信息

Enferm Infecc Microbiol Clin. 1989 Jun-Jul;7(6):316-20.

Abstract

In the present prospective study we have evaluated the sensitivity, specificity and predictive value of the Bengal rose and indirect immunofluorescence (IIF) in 122 patients with a bacteriological diagnosis of brucellosis. The sensitivity of the Bengal rose was 95.79% and its specificity 98.43%. IIF had a sensitivity of 68.80% and a specificity of 100% when the polyvalent anti-IgS conjugate was used. The combined parallel use of Bengal rose and IIF-IgS resulted in a sensitivity of 89.17% and a specificity of 100%. There was a good correlation between Bengal rose and serum agglutination test (r = 0.72); on the contrary, there were not good correlations between the fluorescent conjugates and Bengal rose, agglutination and Coombs test. In conclusion, the combined use of Bengal rose as a screening test and IIF as a confirmation study is a good diagnostic strategy for human brucellosis.

摘要

在本前瞻性研究中，我们评估了孟加拉玫瑰红染色和间接免疫荧光法（IIF）在122例经细菌学诊断为布鲁氏菌病患者中的敏感性、特异性和预测价值。孟加拉玫瑰红染色的敏感性为95.79%，特异性为98.43%。当使用多价抗IgS结合物时，IIF的敏感性为68.80%，特异性为100%。孟加拉玫瑰红染色和IIF-IgS联合平行使用时，敏感性为89.17%，特异性为100%。孟加拉玫瑰红染色与血清凝集试验之间存在良好的相关性（r = 0.72）；相反，荧光结合物与孟加拉玫瑰红染色、凝集试验和库姆斯试验之间没有良好的相关性。总之，将孟加拉玫瑰红染色作为筛查试验，IIF作为确诊研究联合使用，是诊断人类布鲁氏菌病的良好策略。