Zhu Jie, Zheng Zhibao, Wang Jia, Sun Jinhua, Wang Pan, Cheng Xianying, Fu Lun, Zhang Liming, Wang Zuojun, Li Zhaoyun
Clinical laboratory, Taizhou Central Hospital, Taizhou, Zhejiang, China Zhejiang, China.
Department of Oncology, Taizhou Central Hospital, Taizhou Zhejiang, China.
Front Genet. 2014 May 27;5:149. doi: 10.3389/fgene.2014.00149. eCollection 2014.
A bunch of microRNAs (miRNAs) have been demonstrated to be aberrantly expressed in cancer tumor tissue and serum. The miRNA signatures identified from the serum samples could serve as potential noninvasive diagnostic markers for breast cancer. The role of the miRNAs in cancerigenesis is unclear. In this study, we generated the expression profiles of miRNAs from the paired breast cancer tumors, normal, tissue, and serum samples from eight patients using small RNA-sequencing. Serum samples from eight healthy individuals were used as normal controls. We identified total 174 significantly differentially expressed miRNAs between tumors and the normal tissues, and 109 miRNAs between serum from patients and serum from healthy individuals. There are only 10 common miRNAs. This suggests that only a small portion of tumor miRNAs are released into serum selectively. Interestingly, the expression change pattern of 28 miRNAs is opposite between breast cancer tumors and serum. Functional analysis shows that the differentially expressed miRNAs and their target genes form a complex interaction network affecting many biological processes and involving in many types of cancer such as prostate cancer, basal cell carcinoma, acute myeloid leukemia, and more.
大量微小RNA（miRNA）已被证明在癌症肿瘤组织和血清中表达异常。从血清样本中鉴定出的miRNA特征可作为乳腺癌潜在的非侵入性诊断标志物。miRNA在癌症发生中的作用尚不清楚。在本研究中，我们使用小RNA测序技术，生成了来自8例患者的配对乳腺癌肿瘤、正常组织和血清样本的miRNA表达谱。来自8名健康个体的血清样本用作正常对照。我们在肿瘤与正常组织之间共鉴定出174个显著差异表达的miRNA，在患者血清与健康个体血清之间鉴定出109个miRNA。只有10个共同的miRNA。这表明只有一小部分肿瘤miRNA被选择性地释放到血清中。有趣的是，28个miRNA在乳腺癌肿瘤和血清中的表达变化模式相反。功能分析表明，差异表达的miRNA及其靶基因形成了一个复杂的相互作用网络，影响许多生物学过程，并涉及多种癌症，如前列腺癌、基底细胞癌、急性髓细胞白血病等。
