GenoType(®) MTBC 分子基因检测与培养方法在结核病诊断中的比较。
Comparison of the GenoType(®) MTBC Molecular Genetic Assay with culture methods in the diagnosis of tuberculosis.
机构信息
Department of Microbiology and Clinical Microbiology, Konya Education and Research Hospital, Konya, Turkey.
Department of Microbiology and Clinical Microbiology, Necmettin Erbakan University Meram Faculty of Medicine, Konya, Turkey.
出版信息
Arch Med Sci. 2014 May 12;10(2):315-8. doi: 10.5114/aoms.2012.29216. Epub 2012 Jun 28.
INTRODUCTION
Clinical samples from 433 patients pre-diagnosed with tuberculosis in Konya, Turkey, were investigated prospectively to compare the GenoType(®) MTBC test (GenoType(®) MTBC) with conventional "gold standard" culture methods.
MATERIAL AND METHODS
Lowenstein Jensen (LJ) and Mycobacteria Growth Indicator Tube (MGIT)-960 culture methods and GenoType(®) MTBC were performed together.
RESULTS
Mycobacterium tuberculosis (M. tuberculosis) detection rates were 16.2% by culture methods, 15.4% by GenoType(®) MTBC, and 6% by acid-fast bacilli microscopy. The LJ or MGIT-960 with GenoType(®) MTBC detected M. tuberculosis in 12 samples each that were negative according to the other culture method alone. Among 70 M. tuberculosis-positive samples, detection rates were 37% (26/70) by microscopy and 82.8% (58/70) by LJ and MGIT-960, but 95.7% (67/70) by GenoType(®) MTBC.
CONCLUSIONS
GenoType(®) MTBC may be used as a beneficial adjunct test to culture methods for the detection of M. tuberculosis.
简介
前瞻性研究了来自土耳其科尼亚的 433 名预先诊断为结核病的患者的临床样本，以比较 GenoType(®) MTBC 检测（GenoType(®) MTBC）与传统的“金标准”培养方法。
材料和方法
同时进行 Lowenstein Jensen（LJ）和分枝杆菌生长指示剂管（MGIT）-960 培养方法和 GenoType(®) MTBC。
结果
培养方法的结核分枝杆菌（M. tuberculosis）检出率为 16.2%，GenoType(®) MTBC 为 15.4%，抗酸杆菌镜检为 6%。LJ 或 MGIT-960 与 GenoType(®) MTBC 共同检测到 12 份单独用其他培养方法检测为阴性的 M. tuberculosis 。在 70 份 M. tuberculosis 阳性样本中，显微镜检测率为 37%（26/70），LJ 和 MGIT-960 检测率为 82.8%（58/70），但 GenoType(®) MTBC 检测率为 95.7%（67/70）。
结论
GenoType(®) MTBC 可作为培养方法检测 M. tuberculosis 的有益辅助检测方法。
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