Ninet B, Rohner P, Metral C, Auckenthaler R
Division of Infectious Diseases, Laboratory of Bacteriology, Hôpital Cantonal Universitaire, CH 1211 Geneva, Switzerland.
J Clin Microbiol. 1999 Mar;37(3):782-4. doi: 10.1128/JCM.37.3.782-784.1999.
The use of the COBAS AMPLICOR System (Roche Molecular Diagnostics, Basel, Switzerland), the only automated system for PCR testing, was evaluated for a rapid identification of mycobacteria with positive BACTEC 12B cultures. Two hundred ninety-six specimens with a growth index of >/=30 were analyzed for the presence of Mycobacterium tuberculosis complex, Mycobacterium avium, and Mycobacterium intracellulare. Compared to traditional methods and provided that samples with PCR inhibition are retested at a 1:10 dilution, the sensitivity and specificity of the COBAS AMPLICOR System with BACTEC 12B cultures were 100 and 98%, respectively. The COBAS AMPLICOR method is rapid and reliable for identifying the most common mycobacteria in cultures.
对唯一的PCR检测自动化系统COBAS AMPLICOR系统（罗氏分子诊断公司，瑞士巴塞尔）进行了评估，以快速鉴定BACTEC 12B培养阳性的分枝杆菌。对296份生长指数≥30的标本进行分析，检测是否存在结核分枝杆菌复合群、鸟分枝杆菌和胞内分枝杆菌。与传统方法相比，且前提是对有PCR抑制作用样本以1:10稀释度重新检测，COBAS AMPLICOR系统对BACTEC 12B培养物检测的灵敏度和特异性分别为100%和98%。COBAS AMPLICOR方法在鉴定培养物中最常见分枝杆菌方面快速且可靠。
