Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Zephase Therapeutics (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India.
Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Zephase Therapeutics (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India.
Pharmacol Rep. 2014 Feb;66(1):179-83. doi: 10.1016/j.pharep.2013.06.012. Epub 2014 Feb 1.
We describe a method for obtaining pharmacokinetics (PK) and pharmacology data from adult zebrafish in terms of mg/kg using a novel method of oral administration. Using carbamazepine (CBZ) as a test drug, we employed dried blood spot (DBS) cards to enable drug quantification for PK; and we evaluated the pharmacological anxiolytic effect using novel tank test.
The PK study confirmed the presence of CBZ in both blood and brain and the behavioural study showed dose dependent anxiolytic effect. The reproducibility of oral dosing was confirmed by the fact that the results obtained in both the experiments had negligible errors.
This report enables a novel approach for optimizing the utility of zebrafish in drug discovery and drug delivery research.
我们描述了一种使用新型口服给药方法，以毫克/千克为单位，从成年斑马鱼中获得药代动力学（PK）和药理学数据的方法。我们使用卡马西平（CBZ）作为测试药物，采用干血斑（DBS）卡进行 PK 药物定量；并采用新型坦克测试评估其抗焦虑的药理作用。
PK 研究证实了 CBZ 存在于血液和大脑中，行为研究表明存在剂量依赖性的抗焦虑作用。这两项实验结果几乎没有误差，这证实了口服给药的重现性。
本报告为优化斑马鱼在药物发现和药物输送研究中的应用提供了一种新方法。
