Görög S, Rényei M, Herényi B
Chemical Works of Gedeon Richter Ltd, Budapest, Hungary.
J Pharm Biomed Anal. 1989;7(12):1527-33. doi: 10.1016/0731-7085(89)80161-x.
New applications in the identification of minor components in drugs by rapid scanning diode-array UV spectrophotometers as high-performance liquid chromatographic (HPLC) detectors are exemplified by (a) identification and quantification of alpha-chloro-4-methoxycinnamic acid methyl ester as the byproduct of the Darzens reaction between 4-methoxybenzaldehyde and chloroacetic acid methyl ester; and (b) identification of 4-androsten-17-one and 3 beta-phenyl-5 alpha-androstan-17-one as impurities in 5 alpha-androst-2-en-17-one. The advantages of the use of derivative spectrophotometry are illustrated by the following examples: (a) determination of flumecinol (3-trifluoromethyl-alpha-ethylbenzhydrol) in an oily emulsion formulation; (b) determination of RGH-6148 (2-benzylthiazolidinone) in a suspension used in toxicological studies; and (c) determination of mestranol as an impurity in norethisterone.
用快速扫描二极管阵列紫外分光光度计作为高效液相色谱（HPLC）检测器来鉴定药物中的次要成分的新应用，体现在以下方面：（a）鉴定和定量α-氯-4-甲氧基肉桂酸甲酯，其为4-甲氧基苯甲醛与氯乙酸甲酯之间的达森斯反应的副产物；（b）鉴定4-雄甾烯-17-酮和3β-苯基-5α-雄甾烷-17-酮为5α-雄甾-2-烯-17-酮中的杂质。以下例子说明了使用导数分光光度法的优点：（a）测定油性乳剂制剂中的氟甲西诺（3-三氟甲基-α-乙基二苯甲醇）；（b）测定毒理学研究中使用的混悬液中的RGH-6148（2-苄基噻唑烷酮）；以及（c）测定炔诺酮中的杂质炔雌醇甲醚。
