Mijušković Ana, Oreščanin-Dušić Zorana, Nikolić-Kokić Aleksandra, Slavić Marija, Spasić Mihajlo B, Spasojević Ivan, Blagojević Duško
Department of Physiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
Department of Physiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
Pharmacol Rep. 2014 Jun;66(3):373-9. doi: 10.1016/j.pharep.2013.12.012. Epub 2014 Apr 13.
Our aim was to investigate the effect of methanethiol (CH3SH) on contractility of rat uterus and activities of redox-active enzymes, and to compare them with the effect of sodium sulphide (Na2S), a hydrogen sulphide (H2S/HS(-)) donor.
Uteri were isolated from virgin Wistar rats, divided into six groups, controls (untreated uteri allowed to contract spontaneously and in the presence of Ca(2+)(6mM)), CH3SH treated (spontaneously active and Ca(2+) induced) and Na2S treated (spontaneously active and Ca(2+) induced). Underlying antioxidative enzyme activities (superoxide dismutase--SOD, glutathione peroxidase--GSHPx, glutathione reductase--GR) in CH3SH- or Na2S-treated uteri were compared to controls.
Our experiments showed that CH3SH and Na2S provoked reversible relaxation of both spontaneous and Ca(2+)-induced uterine contractions. The dose-response curves differed in shape, and CH3SH curve was shifted to higher concentration compared to H2S/HS(-). The effects of Na2S fitted sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked increased SOD activity and decreased GR activity. However, Na2S (H2S/HS(-)) provoked an increase in SOD activity exclusively in Ca(2+)-stimulated uteri, while the activity of GSHPx was increased in both types of active uteri.
Our results imply that CH3SH may have a constructive role in the control of muscle function and metabolism. Observed differences between CH3SH and H2S/HS(-) could be attributed to a larger moiety that is present in CH3SH compared to H2S, but they are more likely to be a consequence of the specific actions of HS(-), in relation to its negative charge.
我们的目的是研究甲硫醇（CH3SH）对大鼠子宫收缩性和氧化还原活性酶活性的影响，并将其与硫化氢（H2S/HS(-)）供体硫化钠（Na2S）的作用进行比较。
从未孕的Wistar大鼠中分离出子宫，分为六组，即对照组（未处理的子宫，可自发收缩并在存在Ca(2+)(6mM)的情况下收缩）、CH3SH处理组（自发活跃和Ca(2+)诱导）和Na2S处理组（自发活跃和Ca(2+)诱导）。将CH3SH或Na2S处理的子宫中的基础抗氧化酶活性（超氧化物歧化酶-SOD、谷胱甘肽过氧化物酶-GSHPx、谷胱甘肽还原酶-GR）与对照组进行比较。
我们的实验表明，CH3SH和Na2S均可引起自发和Ca(2+)诱导的子宫收缩的可逆性松弛。剂量-反应曲线形状不同，与H2S/HS(-)相比，CH3SH曲线向更高浓度偏移。Na2S的作用符合S形曲线，而CH3SH的作用呈线性。CH3SH可引起SOD活性增加和GR活性降低。然而，Na2S（H2S/HS(-)）仅在Ca(2+)刺激的子宫中引起SOD活性增加，而在两种活跃子宫中GSHPx活性均增加。
我们的结果表明，CH3SH可能在肌肉功能和代谢的控制中具有建设性作用。CH3SH与H2S/HS(-)之间观察到的差异可能归因于与H2S相比CH3SH中存在的较大部分，但它们更可能是HS(-)特定作用的结果，与其负电荷有关。
