Gołębiowski Marek, Cerkowniak Magdalena, Urbanek Aleksandra, Słocińska Małgorzata, Rosiński Grzegorz, Stepnowski Piotr
Laboratory of Natural Products Analysis, Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdansk, ul. Wita Stwosza 63, 80-308, Poland.
Laboratory of Natural Products Analysis, Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdansk, ul. Wita Stwosza 63, 80-308, Poland.
Peptides. 2014 Aug;58:65-73. doi: 10.1016/j.peptides.2014.05.013. Epub 2014 Jun 4.
In insects, neuropeptide adipokinetic hormone (AKH) released from the corpora cardiaca mobilizes lipids and carbohydrates in the fat body. We examined the developmental differences in the action of Tenmo-AKH, a bioanalogue belonging to the adipokinetic/hypertrahelosemic family (AKH/HrTH), on the lipid composition of larval and pupal fat bodies in the beetle Zophobas atratus. Tenmo-AKH was administered to the beetle larvae and pupae either as a single dose or as two doses of 20 pmol during a 24h interval. Extracts of fat bodies were used to analyse the lipid composition by gas chromatography (GC) combined with mass spectrometry (GC-MS). Control extracts were analyzed using the same method. Fatty acids (FA) and fatty acid methyl esters (FAME) were the most abundant compounds in the fat bodies from both developmental stages. We observed significant differences in their concentrations following hormonal treatment. Tenmo-AKH also induced a distinct increase in larval sterols, fatty alcohols and benzoic acid.
在昆虫中，从心侧体释放的神经肽脂肪动激素（AKH）可动员脂肪体中的脂质和碳水化合物。我们研究了Tenmo - AKH（一种属于脂肪动/高血糖激素家族（AKH/HrTH）的生物类似物）对拟步甲幼虫和蛹脂肪体脂质组成作用的发育差异。将Tenmo - AKH以单剂量或在24小时间隔内分两剂、每剂20皮摩尔的方式施用于拟步甲幼虫和蛹。脂肪体提取物用于通过气相色谱（GC）结合质谱（GC - MS）分析脂质组成。对照提取物采用相同方法进行分析。脂肪酸（FA）和脂肪酸甲酯（FAME）是两个发育阶段脂肪体中含量最丰富的化合物。我们观察到激素处理后它们的浓度存在显著差异。Tenmo - AKH还使幼虫中的固醇、脂肪醇和苯甲酸明显增加。
