Slocinska Malgorzata, Marciniak Pawel, Jarmuszkiewicz Wieslawa, Rosinski Grzegorz
Department of Animal Physiology and Development, Adam Mickiewicz University, Poznań, Poland.
Department of Animal Physiology and Development, Adam Mickiewicz University, Poznań, Poland.
Peptides. 2015 Jun;68:157-63. doi: 10.1016/j.peptides.2014.05.010. Epub 2014 May 29.
Insect sulfakinins are multifunctional neuropeptides homologous to vertebrate gastrin/cholecystokin (CCK) neuropeptide hormones. We investigated the action of the nonsulfated sulfakinin Zopat-SK-1 (pETSDDYGHLRFa) on the levels of chosen metabolites in the Zophobas atratus beetle fat body. Samples of fat body were collected 2h and 24h after hormone injection. The administration of 20pmol of Zopat-SK-1 to feeding larvae significantly increased concentrations of lipids and proteins and decreased the content of glycogen in fat body tissue in the 24h experimental group. In contrast, the only increase in total lipid concentration in prepupal fat bodies was observed 24h after Zopat-SK-1 treatment. Simultaneously, changes in the quality and quantity of free sugars in the hemolymph were measured. In larval hemolymph, a marked increase in free sugar concentration and a decrease in glucose content were observed 24h and 2h after Zopat-SK-1 application, respectively. No changes in the prepupal stage were observed. For the first time we show potent metabolic activity of sulfakinin in the fat body tissue of an insect. Our findings imply a physiological function of the nonsulfated form of sulfakinin in energy storage and release processes in fat body tissue of larvae and prepupae was indicated. We suggest a role for sulfakinin signaling in the regulation of energy metabolism in insect tissues.
昆虫速激肽是与脊椎动物胃泌素/胆囊收缩素（CCK）神经肽激素同源的多功能神经肽。我们研究了非硫酸化速激肽Zopat-SK-1（pETSDDYGHLRFa）对暗黑鳃金龟脂肪体中选定代谢物水平的作用。在激素注射后2小时和24小时收集脂肪体样本。在24小时实验组中，给取食幼虫注射20 pmol的Zopat-SK-1可显著增加脂肪体组织中脂质和蛋白质的浓度，并降低糖原含量。相比之下，在Zopat-SK-1处理24小时后，仅观察到蛹前脂肪体中总脂质浓度增加。同时，测量了血淋巴中游离糖的质量和数量变化。在幼虫血淋巴中，Zopat-SK-1应用后24小时和2小时分别观察到游离糖浓度显著增加和葡萄糖含量降低。在蛹前期未观察到变化。我们首次展示了速激肽在昆虫脂肪体组织中的强大代谢活性。我们的研究结果表明，非硫酸化形式的速激肽在幼虫和蛹前脂肪体组织的能量储存和释放过程中具有生理功能。我们认为速激肽信号在昆虫组织能量代谢调节中发挥作用。
