硅胶柱和氧化铝柱用于在线柱切换固相萃取后碱性药物及血浆成分的高效液相色谱分离的比较
A comparison of silica and alumina columns for high-performance liquid chromatographic separations of basic drugs and plasma constituents following on-line solid-phase extraction with column switching.
作者信息
Kelly M T, Smyth M R
机构信息
School of Chemical Sciences, Dublin City University, Glasnevin, Ireland.
出版信息
J Pharm Biomed Anal. 1989;7(12):1757-64. doi: 10.1016/0731-7085(89)80191-8.
A comparison has been made of a silica and an alumina column with respect to retention behaviour in high-performance liquid chromatography of pure drug solutions and plasma profiles following on-line solid-phase extraction. Using methanol-ammonium nitrate buffer (80:20, v/v) as eluent, it was found that these systems lend themselves well to the column switching technique. Increasing the pH of the mobile phase produced similar shifts in retention for basic drugs on both columns, but fewer plasma interferents are retained on the alumina column as compared to the silica column under the same operating conditions.
对硅胶柱和氧化铝柱在高效液相色谱中纯药物溶液的保留行为以及在线固相萃取后的血浆图谱进行了比较。使用甲醇 - 硝酸铵缓冲液（80:20，v/v）作为洗脱剂，发现这些系统非常适合柱切换技术。增加流动相的pH值会使两种柱上碱性药物的保留发生类似的变化，但在相同操作条件下，与硅胶柱相比，氧化铝柱上保留的血浆干扰物更少。
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