Smith R M, Hurdley T G, Westlake J P, Gill R, Osselton M D
Department of Chemistry, Loughborough University of Technology, U.K.
J Chromatogr. 1988 Nov 25;455:77-93. doi: 10.1016/s0021-9673(01)82108-7.
The effect of changing the brand and batches of the silica stationary phase used with a methanol-aqueous ammonium nitrate eluent for the high-performance liquid chromatographic separation of basic drugs has been studied. Considerable care had to be taken to obtain a reproducible eluent and the effect of small changes in the concentration of the ammonia solution were examined closely. Large differences in both the capacity factor and relative capacity factors were found for separations on columns packed with four different brands of silica. Significant differences were also observed with columns containing eighteen different batches of Spherisorb S5W, which had been manufactured over a period of several years.
研究了更换硅胶固定相的品牌和批次对使用甲醇 - 硝酸铵水溶液洗脱剂进行高效液相色谱分离碱性药物的影响。为了获得可重现的洗脱剂，必须格外小心，并仔细研究氨溶液浓度的微小变化所产生的影响。对于用四种不同品牌硅胶填充的色谱柱进行分离时，容量因子和相对容量因子都存在很大差异。对于含有在几年时间内生产的18个不同批次Spherisorb S5W的色谱柱，也观察到了显著差异。
