Bioelectronics Programme, Institute of Microelectronics, A(⁎)STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore.
Bioelectronics Programme, Institute of Microelectronics, A(⁎)STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore.
Biosens Bioelectron. 2014 Nov 15;61:260-5. doi: 10.1016/j.bios.2014.05.029. Epub 2014 May 22.
A new platform based on 4-Fluoro-3-nitrophenyl (FNP) grafted gold disk electrode prepared via electrochemical reduction of 4-fluoro-3-nitrobenzene diazonium ion has been developed and utilized for biosensor fabrication. Anti-interleukin-2 (anti-IL2) antibody has been covalently immobilized onto FNP/Au surface and utilized for label free electrochemical impedance based detection of cytokine IL2. FNP acts as a bridge (cross-linker) between gold surface and anti-IL2, where fluoro group of FNP undergoes nucleophilic substitution by amino group of biomolecule and results in its covalent immobilization. The immobilization process and fabricated electrode have been characterized using contact angle (CA) measurements, cyclic voltammetry (CV) and electrochemical impedance (EIS) technique. CV studies show that FNP grafted surface provides conductive surface for anti-IL2 immobilization. The EIS response of studies as a function of IL2 concentrations exhibits a detection in linear range from 1 pg ml(-1) to 10 ng ml(-1) with minimum detectable concentration of 1 pg ml(-1). The electrode has been found to be selective against other cytokine molecules.
一个新的平台，基于 4-氟-3-硝基苯基（FNP）接枝金盘电极通过电化学还原 4-氟-3-硝基苯重氮离子制备已开发和利用生物传感器的制造。抗白细胞介素 2（抗 IL2）抗体已共价固定到 FNP/Au 表面，并用于无标记的基于电化学阻抗的细胞因子 IL2 的检测。FNP 作为金表面和抗 IL2 之间的桥梁（交联剂），其中 FNP 的氟原子经历亲核取代的生物分子的氨基，导致其共价固定。该固定化过程和制备的电极的使用接触角（CA）测量，循环伏安法（CV）和电化学阻抗（EIS）技术进行了表征。CV 研究表明，FNP 接枝表面提供了用于抗 IL2 固定化的导电表面。作为 IL2 浓度的函数的 EIS 响应表现出检测线性范围从 1 pg ml(-1)到 10 ng ml(-1)，最低检测浓度为 1 pg ml(-1)。该电极已被发现对其他细胞因子分子具有选择性。
