Peng Jie, Raghuraman M K, Feng Wenyi
Department of Biochemistry & Molecular Biology, SUNY Upstate Medical University, 4287 Weiskotten Hall, 750 East Adams Street, Syracuse, NY, 13210, USA.
Methods Mol Biol. 2014;1170:477-99. doi: 10.1007/978-1-4939-0888-2_26.
Eukaryotic DNA replication exhibits at once extraordinary fidelity and substantial plasticity. The importance of the apparent presence of a replication temporal program on a population level has been the subject of intense debate of late. Such debate has been, to a great extent, facilitated by methods that permit the description and analysis of replication dynamics in various model organisms, both globally and at a single-molecule level. Each of these methods provides a unique view of the replication process, and also presents challenges and questions in the interpretation of experimental observations. Thus, wider applications of these methods in different genetic backgrounds and in different organisms would doubtless enable us to better understand the execution and regulation of chromosomal DNA synthesis as well as its impact on genome maintenance.
真核生物的DNA复制既展现出非凡的保真度，又具有显著的可塑性。近期，在群体水平上明显存在复制时间程序这一现象的重要性引发了激烈的争论。在很大程度上，能够在全球范围内以及单分子水平描述和分析各种模式生物复制动态的方法推动了此类争论。每种方法都为复制过程提供了独特的视角，同时在解释实验观察结果时也带来了挑战和问题。因此，这些方法在不同遗传背景和不同生物体中的更广泛应用无疑将使我们能够更好地理解染色体DNA合成的执行与调控及其对基因组维持的影响。
