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[凝固酶阴性葡萄球菌菌株产黏液研究的两种方法评估]

[Evaluation of 2 methods for studying slime production by coagulase-negative Staphylococcus strains].

作者信息

Sánchez G, Pascual A, Martínez-Martínez L

出版信息

Enferm Infecc Microbiol Clin. 1989 May;7(5):252-6.

PMID:2490721
Abstract

The surface hydrophobicity and the slime production of 46 coagulase negative staphylococci isolated from the urine, blood and skin were evaluated. The hydrophobicity values were very heterogeneous and did not correlate with the species, origin or slime production. Slime production was evaluated by the classical tube method and by a micromethod with spectrophotometric readings. There was a high correlation between both methods, but the results were more reproducible and quantifiable with the latter. Twenty-six of the evaluated species produced some degree of slime. This feature did not correlate with their origin or species. The preincubation of 5 strains of Staphylococcus epidermidis with subinhibitory concentrations of vancomycin, clindamycin and amikacin did not influence slime production, whereas ciprofloxacin slightly inhibited it in two strains and oxacillin completely inhibited slime production in the five evaluated strains.

摘要

对从尿液、血液和皮肤中分离出的46株凝固酶阴性葡萄球菌的表面疏水性和黏液产生情况进行了评估。疏水性值差异很大,且与菌种、来源或黏液产生无关。通过经典试管法和采用分光光度读数的微量法评估黏液产生情况。两种方法之间具有高度相关性,但后者的结果更具可重复性和可量化性。在所评估的菌种中,有26种产生了一定程度的黏液。这一特征与其来源或菌种无关。用亚抑菌浓度的万古霉素、克林霉素和阿米卡星对5株表皮葡萄球菌进行预孵育,并未影响黏液产生,而环丙沙星在两株菌株中轻微抑制了黏液产生,苯唑西林在5株评估菌株中完全抑制了黏液产生。

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