Department of Biology, Federal University of Lavras (UFLA), CP 3037 - Campus Universitário, CEP 37.200-000 Lavras, MG, Brazil.
Department of Biology, Federal University of Lavras (UFLA), CP 3037 - Campus Universitário, CEP 37.200-000 Lavras, MG, Brazil.
Bioresour Technol. 2014 Aug;166:142-50. doi: 10.1016/j.biortech.2014.05.031. Epub 2014 May 20.
The semi-dry processing of coffee generates significant amounts of coffee pulp and wastewater. This study evaluated the production of bioethanol and volatile compounds of eight yeast strains cultivated in a mixture of these residues. Hanseniaspora uvarum UFLA CAF76 showed the best fermentation performance; hence it was selected to evaluate different culture medium compositions and inoculum size. The best results were obtained with 12% w/v of coffee pulp, 1 g/L of yeast extract and 0.3 g/L of inoculum. Using these conditions, fermentation in 1 L of medium was carried out, achieving higher ethanol yield, productivity and efficiency with values of 0.48 g/g, 0.55 g/L h and 94.11% respectively. Twenty-one volatile compounds corresponding to higher alcohols, acetates, terpenes, aldehydes and volatile acids were identified by GC-FID. Such results indicate that coffee residues show an excellent potential as substrates for production of value-added compounds. H. uvarum demonstrated high fermentative capacity using these residues.
咖啡的半干法加工会产生大量的咖啡果肉和废水。本研究评估了在这些残渣混合物中培养的八种酵母菌株生产生物乙醇和挥发性化合物的情况。汉逊酵母(Hanseniaspora uvarum)UFLA CAF76 的发酵性能最佳,因此选择其来评估不同培养基组成和接种量。在咖啡果肉 12%(w/v)、酵母提取物 1 g/L 和接种量 0.3 g/L 的最佳条件下,在 1 L 培养基中进行发酵,可获得 0.48 g/g、0.55 g/L h 和 94.11%的较高乙醇产率、生产力和效率。通过 GC-FID 鉴定出 21 种挥发性化合物,分别为高级醇、乙酸酯、萜烯、醛和挥发性酸。这些结果表明,咖啡残渣作为生产高附加值化合物的底物具有良好的潜力。汉逊酵母(Hanseniaspora uvarum)使用这些残渣具有较高的发酵能力。