Wang Pan, Wang Hui-Ping, Xu Ming-Yuan, Liang Yu-Jie, Sun Ying-Jian, Yang Lin, Li Li, Li Wei, Wu Yi-Jun
Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 1-5 Beichenxi Road, Beijing 100101, PR China.
Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 1-5 Beichenxi Road, Beijing 100101, PR China; Department of Veterinary Medicine and Animal Science, Beijing University of Agriculture, Beijing 102206, PR China.
Food Chem Toxicol. 2014 Aug;70:222-30. doi: 10.1016/j.fct.2014.05.027. Epub 2014 Jun 5.
Organophosphorus (OP) and carbamate (CM) pesticides are widely used in agriculture. These pesticides are highly toxic to humans and their residues in food pose potential threat to human health. In this study, we investigated the effect of subchronic low-level exposure of OPs (dichlorvos, DDVP; malathion, MAL), CM pirimicarb (PI), or their mixtures (DDVP+MAL, DDVP+PI) on mice liver. Metabonomic analysis based on (1)H nuclear magnetic resonance spectroscopy was carried out in combination with biochemical assays. Serum metabonomic analysis showed that levels of trimethylamine-N-oxide, lactate, acetone, very low- and low-density lipoprotein and 3-hydroxybutyrate changed after exposure to the pesticides. In the liver extracts, lactate, glucose, choline, glutathione, alanine, glutamine and isoleucine levels changed after the treatment by pesticides. Our results indicated that exposure to low dose DDVP, MAL and PI, either alone or in combination lead to alteration of liver glucose, fat and protein metabolism, energy metabolism and oxidative balance. This study also showed that metabonomics is of potential use in food toxicity study.
有机磷（OP）和氨基甲酸酯（CM）类农药在农业中广泛使用。这些农药对人类具有高毒性，其在食品中的残留对人类健康构成潜在威胁。在本研究中，我们调查了亚慢性低剂量接触有机磷农药（敌敌畏、DDVP；马拉硫磷、MAL）、氨基甲酸酯类农药抗蚜威（PI）或它们的混合物（DDVP+MAL、DDVP+PI）对小鼠肝脏的影响。结合生化分析，采用基于氢核磁共振波谱的代谢组学分析方法。血清代谢组学分析表明，接触农药后，氧化三甲胺、乳酸、丙酮、极低密度脂蛋白和低密度脂蛋白以及3-羟基丁酸的水平发生了变化。在肝脏提取物中，经农药处理后，乳酸、葡萄糖、胆碱、谷胱甘肽、丙氨酸、谷氨酰胺和异亮氨酸的水平发生了变化。我们的结果表明，低剂量接触DDVP、MAL和PI，单独或联合接触均会导致肝脏葡萄糖、脂肪和蛋白质代谢、能量代谢以及氧化平衡的改变。本研究还表明，代谢组学在食品毒性研究中具有潜在用途。
