Dwivedi Nidhi, Flora Govinder, Kushwaha Pramod, Flora Swaran J S
Division of Regulatory Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002, India.
Department of Applied Sciences, Sant Baba Bhag Singh Institute of Engineering and Technology, Jalandhar 144030, Panjab, India.
Environ Toxicol Pharmacol. 2014 Jan;37(1):7-23. doi: 10.1016/j.etap.2013.10.010. Epub 2013 Oct 24.
We investigated protective efficacy of α-lipoic acid (LA), an antioxidant against arsenic and DDVP co-exposed rats. Biochemical variables suggestive of oxidative stress, neurological dysfunction, and tissue histopathological alterations were determined. Male rats were exposed either to 50 ppm sodium arsenite in drinking water or in combination with DDVP (4 mg/kg, subcutaneously) for 10 weeks. α-Lipoic acid (50mg/kg, pos) was also co-administered in above groups. Arsenic exposure led to significant oxidative stress along, hepatotoxicity, hematotoxicity and altered brain biogenic amines levels accompanied by increased arsenic accumulation in blood and tissues. These altered biochemical variables were supported by histopathological examinations leading to oxidative stress and cell death. These biochemical alterations were significantly restored by co-administration of α-lipoic acid with arsenic and DDVP alone and concomitantly. The results indicate that arsenic and DDVP induced oxidative stress and cholinergic dysfunction can be significantly protected by the supplementation of α-lipoic acid.
我们研究了抗氧化剂α-硫辛酸（LA）对砷和敌敌畏共同暴露大鼠的保护作用。测定了提示氧化应激、神经功能障碍和组织组织病理学改变的生化变量。雄性大鼠在饮用水中暴露于50 ppm亚砷酸钠，或与敌敌畏（4 mg/kg，皮下注射）联合暴露10周。上述组中还共同给予α-硫辛酸（50mg/kg，口服）。砷暴露单独导致显著的氧化应激、肝毒性、血液毒性，并改变脑生物胺水平，同时血液和组织中的砷积累增加。这些改变的生化变量得到了组织病理学检查的支持，导致氧化应激和细胞死亡。单独和同时将α-硫辛酸与砷和敌敌畏共同给药可显著恢复这些生化改变。结果表明，补充α-硫辛酸可显著保护砷和敌敌畏诱导的氧化应激和胆碱能功能障碍。
