Laboratory for Biomechanics and Biomaterials, Department of Orthopedic Surgery, Hannover Medical School, Anna-von-Borries-Straße 1-7, 30625 Hannover, Germany; CrossBIT, Center for Biocompatibility and Implant-Immunology, Department of Orthopedic Surgery, Hannover Medical School, Feodor-Lynen-Str. 31, 30625 Hannover.
Institute of Materials Science, Leibniz Universität Hannover, An der Universität 2, 30823 Garbsen, Germany.
Mater Sci Eng C Mater Biol Appl. 2014 Aug 1;41:267-73. doi: 10.1016/j.msec.2014.04.063. Epub 2014 May 5.
Using several different in vitro assays, a new biodegradable magnesium alloy Mg-2La, composed of 98% magnesium and 2% lanthanum, was investigated as a possible implant material for biomedical applications. An in vitro cytotoxicity test, according to EN ISO 10993-5/12, with L929 and human osteoblastic cells identified no toxic effects on cell viability at physiological concentrations (at 50% dilutions and higher). The metabolic activity of human osteoblasts in the 100% extract was decreased to <70% and was therefore rated as cytotoxic. The degradation rates of Mg-2La were evaluated in phosphate buffered saline and four different cell culture media. The degradation rates were shown to be influenced by the composition of the solution, and the addition of fetal bovine serum slightly accelerated the corrosive process. The results of these in vitro experiments suggest that Mg-2La is a promising candidate for use as an orthopedic implant material.
采用多种不同的体外检测方法，研究了一种新型可生物降解的镁合金 Mg-2La，其组成为 98%的镁和 2%的镧。这种镁合金有望用于生物医学植入物材料。根据 EN ISO 10993-5/12 标准进行的体外细胞毒性试验，用 L929 和人成骨细胞进行检测，在生理浓度下（50%稀释度及以上），未发现对细胞活力有任何毒性作用。100%浸提液中人成骨细胞的代谢活性降低至<70%，因此被评定为具有细胞毒性。在磷酸盐缓冲液和四种不同的细胞培养液中评估了 Mg-2La 的降解速率。结果表明，降解速率受溶液成分的影响，胎牛血清的添加略微加速了腐蚀性过程。这些体外实验的结果表明，Mg-2La 是一种有前途的可用于骨科植入物材料的候选材料。
