Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstrasse 6, 91058 Erlangen, Germany.
Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Martensstrasse 7, 91058 Erlangen, Germany.
Mater Sci Eng C Mater Biol Appl. 2014 Aug 1;41:320-8. doi: 10.1016/j.msec.2014.04.052. Epub 2014 Apr 29.
PHBV microsphere/45S5 bioactive glass (BG) composite scaffolds with drug release function were developed for bone tissue engineering. BG-based glass-ceramic scaffolds with high porosity (94%) and interconnected pore structure prepared by foam replication method were coated with PHBV microspheres (nominal diameter=3.5 μm) produced by water-in-oil-in-water double emulsion solvent evaporation method. A homogeneous microsphere coating throughout the porous structure of scaffolds was obtained by a simple dip coating method, using the slurry of PHBV microspheres in hexane. Compressive strength tests showed that the microsphere coating slightly improved the mechanical properties of the scaffolds. It was confirmed that the microsphere coating did not inhibit the bioactivity of the scaffolds in SBF. Hydroxyapatite crystals homogeneously grew not only on the struts of the scaffolds but also on the surface of microspheres within 7 days of immersion in SBF. Vancomycin was successfully encapsulated into the PHBV microspheres. The encapsulated vancomycin was released with a dual release profile involving a relatively low initial burst release (21%) and a sustained release (1 month), which is favorable compared to the high initial burst release (77%) and short release period (4 days) measured on uncoated scaffolds. The developed bioactive composite scaffold with drug delivery function has thus the potential to be used advantageously in bone tissue engineering.
用于骨组织工程的具有释药功能的 PHBV 微球/45S5 生物活性玻璃（BG）复合支架被开发出来。通过泡沫复制法制备的具有高孔隙率（94%）和互连孔结构的 BG 基玻璃陶瓷支架用通过水包油包水双乳液溶剂蒸发法制备的 PHBV 微球（名义直径=3.5μm）进行涂层。通过简单的浸涂法，使用 PHBV 微球在己烷中的浆料，在支架的多孔结构中获得了均匀的微球涂层。压缩强度测试表明，微球涂层略微改善了支架的机械性能。证实微球涂层并未抑制支架在 SBF 中的生物活性。在 SBF 浸泡 7 天内，羟基磷灰石晶体不仅均匀地生长在支架的支柱上，而且还均匀地生长在微球的表面上。万古霉素成功地封装到 PHBV 微球中。包封的万古霉素以双释放曲线释放，包括相对较低的初始突释（21%）和持续释放（1 个月），与未涂层支架测量的高初始突释（77%）和短释放期（4 天）相比，这是有利的。因此，具有药物输送功能的这种新型生物活性复合支架具有在骨组织工程中有利应用的潜力。
