Rahman A, Hoffman N E, Rustum A M
Chemistry Department, Marquette University, Milwaukee, WI 53233.
J Pharm Biomed Anal. 1989;7(6):747-53. doi: 10.1016/0731-7085(89)80119-0.
A high-performance liquid chromatographic method has been developed for the determination of ranitidine in plasma. Ranitidine was extracted with acetonitrile by adding it to the plasma and then salting it out with potassium carbonate. The chromatographic column was 5-microns ODS silica, the mobile phase being acetonitrile-7 mM triethylammonium ion in phosphoric acid (pH 3.00) (30:70, v/v). The ranitidine peak was monitored at a wavelength of 315 nm, the retention time for ranitidine being 4.6 min. A limit of detection of 3 ng ml-1 was obtained for a 100-microliters injection of ranitidine. The method was found to be reproducible with a relative standard deviation (RSD) between 0.8-5.3% (n = 5) over the concentration range 25-80 ng ml-1 in plasma. The ranitidine concentration was determined in 18 different patients' plasmas. Ranitidine and its metabolites ranitidine S-oxide, ranitidine N-oxide and desmethyl-ranitidine, were also studied for chromatographic resolution from each other. It was shown that a group of common drugs did not interfere with ranitidine determination.
已开发出一种高效液相色谱法用于测定血浆中的雷尼替丁。将乙腈加入血浆中，然后用碳酸钾盐析，从而提取雷尼替丁。色谱柱为5微米的ODS硅胶，流动相为乙腈 - 7 mM磷酸三乙铵离子（pH 3.00）（30:70，v/v）。在315 nm波长处监测雷尼替丁峰，雷尼替丁的保留时间为4.6分钟。对于100微升的雷尼替丁注射液，检测限为3 ng/ml。发现在血浆浓度范围为25 - 80 ng/ml时，该方法具有重现性，相对标准偏差（RSD）在0.8 - 5.3%之间（n = 5）。测定了18位不同患者血浆中的雷尼替丁浓度。还研究了雷尼替丁及其代谢产物雷尼替丁S - 氧化物、雷尼替丁N - 氧化物和去甲基雷尼替丁之间的色谱分离情况。结果表明，一组常用药物不会干扰雷尼替丁的测定。
