Farthing D, Brouwer K L, Fakhry I, Sica D
Division of Clinical Pharmacology and Hypertension, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0160, USA.
J Chromatogr B Biomed Sci Appl. 1997 Jan 24;688(2):350-3. doi: 10.1016/s0378-4347(96)00175-2.
An improved high-performance liquid chromatographic (HPLC) method utilizing solid-phase extraction (SPE) and midbore chromatography was developed for the determination of ranitidine in human plasma. A mobile phase of 20 mM K2HPO4-acetonitrile-triethylamine (87.9:12.0:0.1, v/v) pH 6.0 was used with a phenyl analytical column and ultraviolet detection (UV). The method demonstrated linearity from 25 to 1000 ng/ml in 500 microliters of plasma with a detection limit of 10 ng/ml. The method was utilized in a pharmacokinetic study evaluating the effects of pancreatico-biliary secretions on ranitidine absorption.
开发了一种改进的高效液相色谱（HPLC）方法，该方法利用固相萃取（SPE）和中径色谱法测定人血浆中的雷尼替丁。使用20 mM磷酸氢二钾-乙腈-三乙胺（87.9:12.0:0.1，v/v）pH 6.0的流动相，搭配苯基分析柱和紫外检测（UV）。该方法在500微升血浆中浓度范围为25至1000 ng/ml时呈线性，检测限为10 ng/ml。该方法用于一项药代动力学研究，评估胰胆分泌对雷尼替丁吸收的影响。
