Brožíčková C, Mikulecká A, Otáhal J
Institute of Physiology Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Physiol Res. 2014;63(5):637-48. doi: 10.33549/physiolres.932781. Epub 2014 Jun 5.
The role of brain derived nitric oxide in the physiology and behavior remains disputable. One of the reasons of the controversies might be systemic side effects of nitric oxide synthase inhibitors. Therefore, under nNOS inhibition by 7-nitroindazole (7-NI) we carried out recordings of blood gasses, blood pressure and spontaneous EEG in conscious adult rats. Locomotion and spontaneous behavior were assessed in an open field. In addition skilled walking and limb coordination were evaluated using a ladder rung walking test. The blood gas analysis revealed a significant increase in pCO(2) 180 min and 240 min after the application of 7-NI. The power and entropy decreased simultaneously with a shift of the mean frequency of the spontaneous EEG toward slow oscillations after 7-NI treatment. The thresholds of evoked potentials underwent a significant drop and a trend towards a slight increase in the I-O curve slope was observed. 7-NI significantly suppressed open field behavior expressed as distance moved, exploratory rearing and grooming. As for the ladder rung walking test the 7-NI treated animals had more errors in foot placement indicating impairment in limb coordination. Therefore our findings suggest that 7-NI increased cortical excitability and altered some physiological and behavioral parameters.
脑源性一氧化氮在生理和行为中的作用仍存在争议。争议的原因之一可能是一氧化氮合酶抑制剂的全身副作用。因此，在7-硝基吲唑（7-NI）抑制神经元型一氧化氮合酶（nNOS）的情况下，我们对成年清醒大鼠进行了血气、血压和自发脑电图记录。在旷场中评估运动和自发行为。此外，使用阶梯行走试验评估熟练行走和肢体协调性。血气分析显示，应用7-NI后180分钟和240分钟时，pCO₂显著升高。7-NI处理后，自发脑电图的功率和熵同时降低，平均频率向慢波振荡偏移。诱发电位阈值显著下降，观察到I-O曲线斜率有轻微增加的趋势。7-NI显著抑制了以移动距离、探索性直立和梳理表示的旷场行为。至于阶梯行走试验，7-NI处理的动物在足部放置上有更多错误，表明肢体协调性受损。因此，我们的研究结果表明，7-NI增加了皮层兴奋性并改变了一些生理和行为参数。
