Mohr Amanda L A, Ofsa Bill, Keil Alyssa Marie, Simon John R, McMullin Matthew, Logan Barry K
The Center for Forensic Science Research and Education, Willow Grove, PA, USA.
NMS Labs, Willow Grove, PA 18901, USA.
J Anal Toxicol. 2014 Sep;38(7):427-31. doi: 10.1093/jat/bku049. Epub 2014 Jun 7.
Ongoing changes in the synthetic cannabinoid drug market create the need for relevant targeted immunoassays for rapid screening of biological samples. We describe the validation and performance characteristics of an enzyme-linked immunosorbent assay designed to detect use of one of the most prevalent synthetic cannabinoids in urine, UR-144, by targeting its pentanoic acid metabolite. Fluorinated UR-144 (XLR-11) has been demonstrated to metabolize to this common product. The assay has significant cross-reactivity with UR-144-5-OH, UR-144-4-OH and XLR-11-4-OH metabolites, but <10% cross-reactivity with the parent compounds, and no measurable cross-reactivity with other synthetic cannabinoids and their metabolites at concentrations of <1,000 ng/mL. The assay's cutoff is 5 ng/mL relative to the pentanoic acid metabolite of UR-144, which is used as the calibrator. The method was validated with 90 positive and negative control urine samples for UR-144, XLR-11 and its metabolites tested versus liquid chromatography-tandem mass spectrometry. The accuracy, sensitivity and specificity were determined to be 100% for the assay at the specified cutoff.
合成大麻素药物市场的持续变化使得需要相关的靶向免疫测定法来快速筛查生物样本。我们描述了一种酶联免疫吸附测定法的验证和性能特征，该测定法旨在通过靶向尿液中最普遍的合成大麻素之一UR-144的戊酸代谢物来检测其使用情况。氟化UR-144（XLR-11）已被证明可代谢为这种常见产物。该测定法与UR-144-5-OH、UR-144-4-OH和XLR-11-4-OH代谢物具有显著的交叉反应性，但与母体化合物的交叉反应性<10%，并且在浓度<1000 ng/mL时与其他合成大麻素及其代谢物没有可测量的交叉反应性。该测定法的临界值相对于用作校准物的UR-144的戊酸代谢物为5 ng/mL。该方法用90份针对UR-144、XLR-11及其代谢物的阳性和阴性对照尿液样本与液相色谱-串联质谱法进行了验证。在指定的临界值下，该测定法的准确性、灵敏度和特异性被确定为100%。
