人血清中释放的核细胞因子高迁移率族蛋白B1（HMGB1）的分析。
Analysis of the released nuclear cytokine HMGB1 in human serum.
作者信息
Wang Haichao, Zhao Lin, Li Jianhua, Zhu Shu, Yeung Maggie
机构信息
Laboratory of Emergency Medicine, The Feinstein Institute for Medical Research, North Shore-LIJ Health System, 350 Community Drive, 11030, Manhasset, NY, USA,
出版信息
Methods Mol Biol. 2014;1172:13-25. doi: 10.1007/978-1-4939-0928-5_2.
A ubiquitous nuclear protein, the high-mobility group box 1 (HMGB1), is secreted by activated macrophages/monocytes and leaked passively from injured cells. HMGB1 functions as a mediator of infection- and injury-elicited inflammatory diseases. Here, we describe a semiquantitative immuno-blotting method to measure the released HMGB1 in human serum, in comparison with a commercially available HMGB1 ELISA technique.
一种普遍存在的核蛋白，即高迁移率族蛋白B1（HMGB1），由活化的巨噬细胞/单核细胞分泌，并从受损细胞中被动泄漏。HMGB1作为感染和损伤引发的炎症性疾病的介质发挥作用。在此，我们描述了一种半定量免疫印迹法，用于测量人血清中释放的HMGB1，并与市售的HMGB1 ELISA技术进行比较。
Zotero 插件