Graduate School of Engineering Science and Technology and Interdisciplinary Program of Engineering, National Yunlin University of Science and Technology, Yunlin 64002, Taiwan.
Department of Biomechatronics Engineering, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
Biosensors (Basel). 2023 May 6;13(5):522. doi: 10.3390/bios13050522.
Rapid, sensitive, and reliable detection of high mobility group box 1 (HMGB1) is essential for medical and diagnostic applications due to its important role as a biomarker of chronic inflammation. Here, we report a facile method for the detection of HMGB1 using carboxymethyl dextran (CM-dextran) as a bridge molecule modified on the surface of gold nanoparticles combined with a fiber optic localized surface plasmon resonance (FOLSPR) biosensor. Under optimal conditions, the results showed that the FOLSPR sensor detected HMGB1 with a wide linear range (10 to 10 g/mL), fast response (less than 10 min), and a low detection limit of 43.4 pg/mL (1.7 pM) and high correlation coefficient values (>0.9928). Furthermore, the accurate quantification and reliable validation of kinetic binding events measured by the currently working biosensors are comparable to surface plasmon resonance sensing systems, providing new insights into direct biomarker detection for clinical applications.
快速、灵敏、可靠地检测高迁移率族蛋白 B1（HMGB1）对于医学和诊断应用至关重要，因为它是慢性炎症生物标志物的重要组成部分。在这里，我们报告了一种使用羧甲基葡聚糖（CM-dextran）作为桥分子修饰在金纳米粒子表面的简便方法，结合光纤局部表面等离子体共振（FOLSPR）生物传感器来检测 HMGB1。在最佳条件下，结果表明 FOLSPR 传感器检测 HMGB1 的线性范围较宽（10 至 10 g/mL），响应速度较快（小于 10 分钟），检测限低至 43.4 pg/mL（1.7 pM），相关系数值较高（>0.9928）。此外，当前工作的生物传感器测量的动力学结合事件的准确定量和可靠验证与表面等离子体共振传感系统相当，为临床应用中的直接生物标志物检测提供了新的见解。
