肾移植受者和供者的人类白细胞抗原（HLA）抗原分布情况。

HLA antigen distribution in renal transplant recipients and donors.

作者信息

Tuladhar A, Shrestha S, Raut P P, Bhandari P, Shrestha P

机构信息

Department of Pathology, Aarogya Foundation, Pulchowk, Lalitapur, Nepal.

Department of Nephrology, National Academy of Medical Sciences, Kathmandu, Nepal.

出版信息

J Nepal Health Res Counc. 2013 Sep;11(25):289-92.

PMID:24908533

Abstract

BACKGROUND

HLA typing analysis is important in renal transplant patient. This study is the first report from Nepal to find out distribution of HLA A, B, DR antigen in live related renal transplant recipients and donors from Nepal. The aim of this study was to investigate the distribution of HLA in the live related renal transplant recipients and donors of Nepal.

METHODS

In a retrospective study, HLA patterns were defined in 100 live related renal transplant recipients and donors. One year study is done from June 2011 to May 2012. The study was done by using sequence specific oligonucleotides primers and polymerase chain reaction and assay. Allele frequencies were obtained by direct counting.

RESULTS

A total of 12 HLA-A, 15 HLA-B and 13 HLA-DRB1 alleles were identified at the four-digit level in the live related renal transplant recipients and donors of Nepal. High frequency alleles were HLA-A11 (34.5%), A24 (17%), A33 (13%); HLA-B15(27%), B35(19%), B40 (10%); HLA-DRB115 (33.5%), DRB112 (21.4%) and DRB1*04 (7.32%).

CONCLUSIONS

These results showed considerable heterogeneity in both HLA class I and class II antigens. To reduce the risk of allograft rejection, transplantation from HLA matched donors is recommended.

摘要

背景

HLA分型分析在肾移植患者中很重要。本研究是尼泊尔的首份报告，旨在查明尼泊尔活体亲属肾移植受者和供者中HLA A、B、DR抗原的分布情况。本研究的目的是调查尼泊尔活体亲属肾移植受者和供者中HLA的分布情况。

方法

在一项回顾性研究中，对100名活体亲属肾移植受者和供者的HLA模式进行了定义。研究时间为2011年6月至2012年5月，为期一年。该研究采用序列特异性寡核苷酸引物和聚合酶链反应及检测方法进行。通过直接计数获得等位基因频率。

结果

在尼泊尔活体亲属肾移植受者和供者中，共鉴定出12个四位水平的HLA-A、15个HLA-B和13个HLA-DRB1等位基因。高频等位基因为HLA-A11（34.5%）、A24（17%）、A33（13%）；HLA-B15（27%）、B35（19%）、B40（10%）；HLA-DRB115（33.5%）、DRB112（21.4%）和DRB1*04（7.32%）。