Thummuri Dinesh, Jeengar Manish Kumar, Shrivastava Shweta, Areti Aparna, Yerra Veera Ganesh, Yamjala Samyuktha, Komirishetty Prashanth, Naidu V G M, Kumar Ashutosh, Sistla Ramakrishna
Department of Pharmacology & Toxicology, National Institute of Pharmaceutical Education & Research, Balanagar, Hyderabad, Andhra Pradesh 500 037, India.
Department of Pharmacology & Toxicology, National Institute of Pharmaceutical Education & Research, Balanagar, Hyderabad, Andhra Pradesh 500 037, India.
Environ Toxicol Pharmacol. 2014 Jul;38(1):58-70. doi: 10.1016/j.etap.2014.05.002. Epub 2014 May 14.
The present study was aimed to evaluvate the apoptogenic potential of ethanolic extract of leaves from Boswellia ovalifoliolata (BL EthOH) and to unravel the molecular mechanisms implicated in apoptosis of Triple Negative Breast Cancer (TNBC) cells. BL EthOH was cytotoxic against TNBC cells like MDA-MB-231 and MDA-MB-453 with IC₅₀ concentrations 67.48 ± 5.45 and 70.03 ± 4.76 μg/ml, respectively. Apoptotic studies showed that BL EthOH was able to induce apoptosis and western blot studies demonstrated that BL EthOH significantly decreased the Phospho-NF-κB (ser536), PCNA, anti-apoptotic protein Bcl-2 expression and increased the expression of pro-apoptotic protein Bax, in MDA-MB-231 and MDA-MB-453 cell lines when compared with untreated cells. Besides, BL EthOH has synergistic chemosensitizing effects on TNBC cells and increased the cytotoxicity of doxorubicin and cisplatin.
本研究旨在评估椭圆叶乳香树叶乙醇提取物（BL EthOH）的凋亡诱导潜力，并揭示三阴乳腺癌（TNBC）细胞凋亡所涉及的分子机制。BL EthOH对MDA-MB-231和MDA-MB-453等TNBC细胞具有细胞毒性，其IC₅₀浓度分别为67.48±5.45和70.03±4.76μg/ml。凋亡研究表明，BL EthOH能够诱导凋亡，蛋白质免疫印迹研究表明，与未处理的细胞相比，BL EthOH显著降低了MDA-MB-231和MDA-MB-453细胞系中磷酸化核因子κB（ser536）、增殖细胞核抗原（PCNA）、抗凋亡蛋白Bcl-2的表达，并增加了促凋亡蛋白Bax的表达。此外，BL EthOH对TNBC细胞具有协同化学增敏作用，并增强了阿霉素和顺铂的细胞毒性。
