Kohoude Midéko Justin, Gbaguidi Fernand, Agbani Pierre, Ayedoun Marc-Abel, Cazaux Sylvie, Bouajila Jalloul
a Laboratoire des IMRCP UMR CNRS 5623, Faculté de pharmacie de Toulouse, Université de Toulouse , Toulouse , France.
b Laboratoire de Pharmacognosie, Center Béninois de la Recherche Scientifique et Technique (CBRST), Université Paul-Sabatier , Porto-Novo , Benin.
Pharm Biol. 2017 Dec;55(1):33-42. doi: 10.1080/13880209.2016.1226356. Epub 2016 Sep 21.
Boswellia dalzielii Hutch. (Burseraceae) is an aromatic plant. The leaves are used for beverage flavouring.
This study investigates the chemical composition and biological activities of various extracts.
The essential oil was prepared via hydrodistillation. Identification and quantification were realized via GC-MS and GC-FID. Consecutive extractions (cyclohexane, dichloromethane, ethyl acetate and methanol) were carried out and various chemical groups (phenolics, flavonoids, tannins, antocyanins and sugar) were quantified. The volatile compounds of organic extracts were identified before and after derivatization. Antioxidant, antihyperuricemia, anti-Alzheimer, anti-inflammatory and anticancer activities were evaluated.
In the essential oil, 50 compounds were identified, including 3-carene (27.72%) and α-pinene (15.18%). 2,5-Dihydroxy acetophenone and β-d-xylopyranose were identified in the methanol extract. Higher phenolic (315.97 g GAE/kg dry mass) and flavonoid (37.19 g QE/kg dry mass) contents were observed in the methanol extract. The methanol extract has presented remarkable IC=6.10 mg/L for antiDPPH, 35.10 mg/L for antixanthine oxidase and 28.01 mg/L for anti-5-lipoxygenase. For acetylcholinesterase inhibition, the best IC (76.20 and 67.10 mg/L) were observed, respectively, with an ethyl acetate extract and the essential oil. At 50 mg/L, the dichloromethane extract inhibited OVCAR-3 cell lines by 65.10%, while cyclohexane extract inhibited IGROV-1 cell lines by 92.60%.
Biological activities were fully correlated with the chemical groups of the extracts. The ethyl acetate and methanol extracts could be considered as potential alternatives for use in dietary supplements for the prevention or treatment of diseases because of these extracts natural antioxidant, antihyperuricemic and anti-inflammatory activities.
达氏乳香（Boswellia dalzielii Hutch.，橄榄科）是一种芳香植物。其叶子用于饮料调味。
本研究调查各种提取物的化学成分和生物活性。
通过水蒸馏制备精油。通过气相色谱 - 质谱联用仪（GC - MS）和气相色谱 - 火焰离子化检测器（GC - FID）进行鉴定和定量分析。进行连续萃取（环己烷、二氯甲烷、乙酸乙酯和甲醇），并对各种化学基团（酚类、黄酮类、单宁、花青素和糖类）进行定量分析。对有机提取物的挥发性化合物在衍生化前后进行鉴定。评估抗氧化、抗高尿酸血症、抗阿尔茨海默病、抗炎和抗癌活性。
在精油中，鉴定出50种化合物，包括3 - 蒈烯（27.72%）和α - 蒎烯（15.18%）。在甲醇提取物中鉴定出2,5 - 二羟基苯乙酮和β - D - 吡喃木糖。甲醇提取物中观察到较高的酚类含量（315.97 g没食子酸当量/kg干质量）和黄酮类含量（37.19 g芦丁当量/kg干质量）。甲醇提取物对DPPH自由基清除能力的IC₅₀为6.10 mg/L，对黄嘌呤氧化酶抑制能力的IC₅₀为35.10 mg/L，对5 - 脂氧合酶抑制能力的IC₅₀为28.01 mg/L。对于乙酰胆碱酯酶抑制，乙酸乙酯提取物和精油分别观察到最佳的IC₅₀（76.20和67.10 mg/L）。在50 mg/L时，二氯甲烷提取物对OVCAR - 3细胞系的抑制率为65.10%，而环己烷提取物对IGROV - 1细胞系的抑制率为92.60%。
生物活性与提取物的化学基团完全相关。由于乙酸乙酯和甲醇提取物具有天然抗氧化、抗高尿酸血症和抗炎活性，可被视为用于预防或治疗疾病的膳食补充剂的潜在替代品。
