Jones Tia M, Lutz Eric A
J Environ Health. 2014 May;76(9):16-20.
The study described in this article evaluated surface survivability of culturable Pseudomonas aeruginosa by time and type (glass, stainless steel, and laminate) using two sampling techniques: contact plates and surface swabs. Recovery of P. aeruginosa decreased logarithmically over time and varied by surface type. P. aeruginosa survival averaged 3.75, 5.75, and 6.75 hours on laminate, glass, and stainless steel, respectively. Culturable P. aeruginosa loss on stainless steel and glass were not different (p > .05); however, laminate had significantly greater loss at each time point than either glass or stainless (p < .05). A comparison of surface swab and contact plate collection efficiencies found no significant difference for laminate surfaces. Swabs, however, had a higher collection efficiency than contact plates (p < .05). For the first time, the authors report P. aeruginosa mean survival time of 3.75-6.75 hours on clinically relevant surfaces, with P. aeruginosa on stainless steel surviving the longest. Their data also indicate that culturable surface sampling appears to most accurately represent actual P. aeruginosa surface loading when swab sampling is used.
本文所述研究采用接触平板和表面拭子两种采样技术，按时间和类型（玻璃、不锈钢和层压板）评估了可培养铜绿假单胞菌的表面存活能力。铜绿假单胞菌的回收率随时间呈对数下降，且因表面类型而异。铜绿假单胞菌在层压板、玻璃和不锈钢表面的存活时间平均分别为3.75小时、5.75小时和6.75小时。不锈钢和玻璃表面可培养铜绿假单胞菌的损失无差异（p>.05）；然而，在每个时间点，层压板的损失均显著大于玻璃或不锈钢（p<.05）。表面拭子和接触平板采集效率的比较发现，层压板表面两者无显著差异。然而，拭子的采集效率高于接触平板（p<.05）。作者首次报告了铜绿假单胞菌在临床相关表面的平均存活时间为3.75 - 6.75小时，其中不锈钢表面的铜绿假单胞菌存活时间最长。他们的数据还表明，当使用拭子采样时，可培养表面采样似乎最能准确代表实际的铜绿假单胞菌表面载量。
